1：Experimental Design and Method Selection:

The study involved synthesizing amino-functionalized mesoporous silica nanoparticles (MSN) conjugated with rose bengal (RB) as a photosensitizer for antimicrobial photodynamic therapy (aPDT) against Candida albicans. Methods included functionalization, conjugation, characterization (UV-Vis, FTIR, fluorescent spectroscopy, HRTEM), dye loading and release studies, cellular uptake, aPDT experiments on planktonic cells and biofilms, ROS detection, cytoplasmic leakage, DNA damage, lipid peroxidation assays, biofilm inhibition, cell viability, EPS quantification, and confocal laser scanning microscopy (CLSM).

2：Sample Selection and Data Sources:

Candida albicans MTCC 227 strain was used, cultured in YPD media. Samples included functionalized MSN, RB, and MSN-RB conjugate.

3：List of Experimental Equipment and Materials:

Equipment included FTIR spectroscope (Thermo Nicolet Model 6700), UV-Vis spectrophotometer (Varian Carry 5000 model), spectrofluorometer (Jobin Yvon Model: FLUOROLOG - FL3-11), HRTEM, laser source (50 mW, 532 nm), microtitre plates, centrifuges, incubators, CLSM (LSM 710, Carl Zeiss Microscopy GMBH). Materials included mesoporous silica nanoparticles (MSN), 3-Aminopropyltrimethoxysilane (APTES), rose bengal (RB), ethanol, PBS buffer, YPD media, and various chemicals for assays.

4：Experimental Procedures and Operational Workflow:

Steps included amino functionalization of MSN, preparation of MSN-RB conjugate, characterization, dye loading and release studies, cellular uptake experiments, aPDT on planktonic cells and biofilms with light irradiation (5 min, 532 nm laser), various assays (ROS detection, cytoplasmic leakage, DNA damage, lipid peroxidation, biofilm inhibition, cell viability, EPS quantification), and CLSM analysis. All experiments were performed in triplicate with statistical analysis.

5：Data Analysis Methods:

Data were analyzed using one-way ANOVA with Tukey test, with p ≤ 0.05 considered significant. Standard curves were used for quantification in UV-Vis and other assays.