1：Experimental Design and Method Selection:

The study involved preparing PB nanospheres via one-pot synthesis and embedding them in a thermosensitive Pluronic F127 hydrogel matrix for peritumoral administration. Methods included TEM, particle sizing, serum stability tests, photothermal performance assessments with 808 nm laser irradiation, rheological measurements, in vitro cell viability assays (MTT method), fluorescence microscopy, in vivo retention studies using DiR labeling, and in vivo anti-cancer efficacy and toxicity evaluations in a tumor-bearing mouse model.

2：Sample Selection and Data Sources:

PB nanospheres were synthesized using polyvinylpyrrolidone and K3Fe(CN)6. Hydrogels were prepared with Pluronic F127. In vitro studies used 4T1 mouse breast cancer cells and L02 hepatic cells. In vivo studies used BALB/c mice with subcutaneously implanted 4T1 cancer cells.

3：Hydrogels were prepared with Pluronic FIn vitro studies used 4T1 mouse breast cancer cells and L02 hepatic cells. In vivo studies used BALB/c mice with subcutaneously implanted 4T1 cancer cells. List of Experimental Equipment and Materials:

3. List of Experimental Equipment and Materials: Transmission electron microscopy (TEM, 100 kV), particle-sizing system (Malvern, UK), infrared imaging camera (Fotric), scanning electron microscopy (3.0 kV), rheometer, fluorescence microscopy, near-infrared imaging system (Berthold), MTT assay kit, propidium iodide, Hoechst 33342, DiR dye, Pluronic F127, polyvinylpyrrolidone (MW 24,000), K3Fe(CN)6, HCl, and various cell culture reagents.

4：0 kV), rheometer, fluorescence microscopy, near-infrared imaging system (Berthold), MTT assay kit, propidium iodide, Hoechst 33342, DiR dye, Pluronic F127, polyvinylpyrrolidone (MW 24,000), K3Fe(CN)6, HCl, and various cell culture reagents. Experimental Procedures and Operational Workflow:

4. Experimental Procedures and Operational Workflow: PB nanospheres were prepared and characterized for size, stability, and photothermal properties. Hydrogels were formulated and assessed for morphology, photothermal performance, rheology, and stability. In vitro cell studies involved treating cells with nanospheres or hydrogels, laser irradiation, and viability/staining assays. In vivo studies included peritumoral injection of hydrogels, laser irradiation, temperature monitoring, tumor measurement, and toxicity assessment via body weight and histology.

5：Data Analysis Methods:

Data were expressed as mean ± SD and analyzed using two-tailed Student's t-test. Similarity of heating curves was evaluated using the f2 factor. Statistical significance was set at P < 0.05.