研究目的
To develop a sensitive and selective electrochemiluminescence aptasensor for multiplexed detection of kanamycin and neomycin antibiotics in food samples using a dual gears mechanism with a single luminophore.
研究成果
The developed ECL aptasensor is sensitive, selective, and reproducible for multiplexed detection of kanamycin and neomycin with wide linear ranges and low detection limits. It successfully applies to real food samples with good recoveries, demonstrating its potential for food safety monitoring. Future work could focus on enhancing stability and expanding to other antibiotics.
研究不足
The method may have limitations in terms of potential interference from other substances in complex food matrices, and the need for careful optimization of parameters such as amounts of materials and scan rates to avoid signal degradation. The sensor's stability over long periods and its applicability to a wider range of samples could be areas for further improvement.
1：Experimental Design and Method Selection:
The study employs a dual gears ECL aptasensing strategy based on MIL-53(Fe)@CdS-PEI as the ECL luminophore, with nanostructural metallic particles (AuNPs or PtNPs) acting as quenchers or enhancers via ERET and SPR processes. The design involves stepwise assembly of DNA gears and aptamers on a modified electrode.
2：Sample Selection and Data Sources:
Milk and honey samples were purchased from a local market in China and spiked with known concentrations of kanamycin and neomycin for analysis.
3：List of Experimental Equipment and Materials:
Includes GCE (glassy carbon electrode), alumina powder for polishing, MIL-53(Fe), CdS QDs, AuNPs, PtNPs, ECD, NHS, BSA, PBS buffer, K2S2O8, and various chemicals for synthesis and modification as detailed in the supporting information.
4：Experimental Procedures and Operational Workflow:
The electrode is polished, modified with MIL-53(Fe)@CdS, followed by activation with ECD/NHS, incubation with gear A, blocking with BSA, and successive incubations with aptamer 1-PtNPs, gear B, and L1. Detection involves incubating with kanamycin or neomycin, rinsing, and measuring ECL signals in PBS with K2S2O8 using an ECL instrument.
5：Detection involves incubating with kanamycin or neomycin, rinsing, and measuring ECL signals in PBS with K2S2O8 using an ECL instrument.
Data Analysis Methods:
5. Data Analysis Methods: ECL intensity is measured and analyzed for linear range, detection limits, reproducibility, and recovery rates using statistical methods such as relative standard deviation (RSD) and calibration curves.
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