1：Experimental Design and Method Selection:

The study involved preparing hydroxy boron nitride nanosheets (OH-BNNS) via a thermal substitution method and decorating them with Pd nanoparticles through a reduction reaction to create Pd@OH-BNNS nanohybrids. These were used as drug carriers for doxorubicin (DOX), with experiments designed to test drug loading, release under various stimuli (pH, glutathione, NIR irradiation), cytotoxicity, cellular uptake, and in vivo antitumor effects.

2：Sample Selection and Data Sources:

MCF-7 human breast cancer cells and S180 tumor-bearing mice were used as models. Samples included OH-BNNS, Pd@OH-BNNS, and Pd@OH-BNNS/DOX nanocomposites.

3：List of Experimental Equipment and Materials:

Equipment included a muffle furnace, ultrasonicator, centrifuge, UV-vis spectrometer, confocal laser scanning microscope (CLSM), infrared thermal camera, and digital microscope. Materials included melamine, boric acid, PdCl2, NaBH4, doxorubicin, phosphate buffer solution (PBS), glutathione (GSH), and cell culture reagents.

4：Experimental Procedures and Operational Workflow:

OH-BNNS were synthesized by calcining g-C3N4 and boric acid, followed by exfoliation. Pd@OH-BNNS was prepared by reducing PdCl2 on OH-BNNS. Drug loading was done by stirring with DOX, and release studies were conducted under varying pH, GSH, and NIR irradiation. Cytotoxicity was assessed via MTT assay, cellular uptake via CLSM, and in vivo studies involved tumor inoculation, treatment injections, and monitoring.

5：Data Analysis Methods:

Data were analyzed using UV-vis spectroscopy for drug quantification, fluorescence measurements, statistical analysis for cell viability and tumor inhibition rates, and imaging analysis for thermal and histological evaluation.