研究目的
To design and synthesize a novel fluorescent sensor for the recognition of copper(II) ions with high sensitivity and selectivity, and to apply it in cell imaging.
研究成果
The sensor CHT effectively recognizes Cu2+ ions with high sensitivity, selectivity, and fast response. It demonstrates low cytotoxicity and successful application in fluorescent imaging of Cu2+ ions in live cells, indicating its potential for biomedical and environmental monitoring.
研究不足
The sensor operates in acetonitrile/PBS buffer mixtures, which may not fully mimic all biological environments. Potential interference in complex real samples was not extensively tested beyond tap water. The cytotoxicity was only assessed up to 20 μM concentrations.
1：Experimental Design and Method Selection:
The sensor CHT was synthesized via a Schiff base reaction between 7-(N,N-diethylamino) coumarin-3-aldehyde and 2-hydrazinobenzothiazole. Its structure was characterized using 1H NMR, 13C NMR, and ESI-MS. UV-vis absorption and fluorescence spectra were measured to study the interaction with Cu2+ ions.
2：Sample Selection and Data Sources:
Solutions were prepared in acetonitrile and PBS buffer (pH=
3：4). Metal ions tested include Mg2+, Zn2+, K+, Na+, Fe3+, Mn2+, Co2+, Ni2+, Ag+, Cd2+, Pb2+, Ca2+, Hg2+. Cell lines used were A549 and MCF-7 cells. List of Experimental Equipment and Materials:
Equipment includes UV-vis spectrophotometer, fluorescence spectrophotometer, ESI-MS, confocal laser scanning microscope, enzyme marker for CCK8 assay. Materials include coumarin derivatives, metal salts, acetonitrile, PBS buffer, cell culture media (1640 and DMEM), fetal bovine serum.
4：Experimental Procedures and Operational Workflow:
Synthesis of CHT, characterization, UV-vis and fluorescence titration with Cu2+ ions, selectivity tests with other metal ions, time-dependent fluorescence experiments, cytotoxicity assay using CCK8, cell imaging with confocal microscopy.
5：Data Analysis Methods:
Fluorescence intensity changes were monitored, recovery studies in tap water were conducted with statistical analysis (RSD), and imaging data were analyzed visually.
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