研究目的
To synthesize and characterize a novel nanocomposite material for X-ray induced photodynamic therapy, focusing on energy transfer between the ZnO:Ga core and PpIX photosensitizer and its ability to generate singlet oxygen.
研究成果
The nanocomposite ZnO:Ga@SiO2-PpIX was successfully synthesized and shows evidence of energy transfer between the core and PpIX, likely nonradiative. It demonstrates the ability to generate singlet oxygen under X-ray irradiation, making it a promising candidate for X-ray induced photodynamic therapy, though further optimization is needed.
研究不足
The energy transfer mechanism is not fully elucidated due to apparatus limitations in measuring very fast decays. The singlet oxygen generation effect is not very strong, indicating room for improvement in synthesis processes such as SiO2 coating and functionalization. The dose rate measurement may have slight variations in suspensions.
1：Experimental Design and Method Selection:
The study involves synthesizing a nanocomposite with a ZnO:Ga scintillating core coated with SiO2 and functionalized with PpIX. Methods include photo-induced synthesis, sol-gel coating, and functionalization procedures. Spectroscopy techniques (steady-state and time-resolved) are used to study luminescence and energy transfer. A commercial chemical probe (APF) is employed to detect singlet oxygen generation under X-ray irradiation.
2：Sample Selection and Data Sources:
Samples include ZnO:Ga nanoparticles, ZnO:Ga@SiO2, and ZnO:Ga@SiO2-PpIX nanocomposites. Data are obtained from synthesized materials using various characterization techniques.
3：List of Experimental Equipment and Materials:
Equipment includes Rigaku MiniFlex 600 diffractometer, JEOL JSM-6510 SEM, Varian Cary 100 spectrophotometer, X-ray tube (Seifert), spectrofluorometer 5000M (Horiba Jobin Yvon), nanoLED sources, and Fricke dosimeter. Materials include zinc oxide, gallium nitrate, formic acid, nitric acid, hydrogen peroxide, silica precursors, PpIX, and APF probe.
4：Experimental Procedures and Operational Workflow:
Synthesis involves irradiating aqueous solutions to form zinc peroxide, annealing, coating with SiO2 via sol-gel, and functionalizing with PpIX. Characterization includes XRD, SEM, absorption and luminescence spectroscopy, and singlet oxygen detection with APF under X-ray irradiation. Dosimetry is performed using Fricke dosimeter.
5：Data Analysis Methods:
Data are analyzed using PDXL2 program for XRD, spectral correction for detection sensitivity, and fitting functions for decay curves. Statistical evaluation is based on spectral overlaps and decay times.
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MiniFlex 600
MiniFlex 600
Rigaku
X-ray diffraction measurements to analyze crystal structure of nanoparticles.
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JSM-6510
JSM-6510
JEOL
Scanning electron microscopy to obtain images of prepared samples.
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Cary 100
Cary 100
Varian
UV-Vis spectrophotometer to measure absorption spectra.
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X-ray tube
Seifert
Excitation source for radioluminescence measurements.
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nanoLED
339 nm and 389 nm
Nanosecond pulse excitation source for photoluminescence decay measurements.
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spectrofluorometer
5000M
Horiba Jobin Yvon
Detection part for luminescence measurements, equipped with monochromator and photon counting detector.
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APF
Invitrogen
Commercial chemical probe to detect singlet oxygen.
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Fricke dosimeter
Dosimetry to measure radiation dose rate.
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vacuum furnace
Clasic 0415 VAC
Annealing of nanoparticles at high temperatures.
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thermoanalyzer
LabSys Evo
Setaram
Annealing under controlled atmosphere.
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