研究目的
To develop a reusable surface plasmon resonance sensor for rapid and sensitive detection of trace copper ions (Cu2+) in water and other samples, addressing the need for easy, inexpensive, and direct monitoring methods.
研究成果
The developed SPR sensor demonstrates excellent stability, reusability, and sensitivity for Cu2+ detection, with a low LOD of 18 ppb. It shows potential for applications in environmental monitoring, food safety, and medical diagnostics, with possibilities for extension to other analytes.
研究不足
The study is limited to Cu2+ detection in controlled laboratory conditions; real-world samples may have interferences. The sensor's reusability was tested up to five elution cycles, and long-term stability in varied environments is not fully explored. The use of EDTA as an eluant, while effective, may involve costs and recovery processes.
1：Experimental Design and Method Selection:
Utilized a four-layer Kretschmann semi-cylindrical prism configuration with a reference beam to enhance sensitivity and stability. Employed intensity modulation SPR sensing with optimized Ag/Au composite film and MCS active layer.
2：Sample Selection and Data Sources:
Prepared Cu2+ analytes by diluting a 1000 ppm standard solution to concentrations of 1-5 ppm. Used deionized water for flushing and EDTA solutions (0.5-10 mmol/L) for elution.
3：5-10 mmol/L) for elution.
List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: He-Ne laser (050-LHP-021, CVI Melles Griot Inc.), chopper (SR540 OPTICAL CHOPPER, Stanford Research Systems Inc.), photodetectors, lock-in amplifier, sapphire semi-cylindrical prism, rotation stage (SGSP-60YAW-W-OB, Sigma Koki Co.), coater (K575XD, Emitech Co.), spin coating instrument, peristaltic pump (BT100-2JDG-1, Longer Precision Pump Co.), flow pool, chitosan powder, glutaraldehyde, acetic acid, EDTA, sapphire substrates, Ag and Au films.
4：Experimental Procedures and Operational Workflow:
Fabricated sensor chips by depositing Ag and Au films on sapphire substrates, then spin-coated MCS film. Conducted stability tests by flushing with DI water and detecting resonance angles. Performed intensity modulation detection for Cu2+ adsorption and elution with EDTA, measuring signal changes over time.
5：Data Analysis Methods:
Analyzed signal response values using linear fitting to determine sensitivity and limit of detection. Used standard deviation calculations for repeatability assessment.
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