研究目的
To develop an ultrasensitive method for detecting kanamycin residue in milk using a SERS-based aptasensor.
研究成果
The SERS-based aptasensor developed is ultrasensitive, with a detection limit of 0.90 pg/mL for kanamycin in milk, showing high selectivity and satisfactory recoveries. It offers a rapid and cost-effective alternative to traditional methods for trace antibiotic detection.
研究不足
The method requires preprocessing of milk samples to remove proteins and fats, which adds complexity. The specificity was tested only against a few interferons (gentamicin, streptomycin, penicillin), and real-world applications might involve more complex matrices.
1：Experimental Design and Method Selection:
The study employed a SERS-based aptasensor using double strand DNA binding bimetallic gold@silver nanoparticles as a sensing platform. Probe DNAs were embedded on gold nanoparticles via thiol modification, followed by silver shell encapsulation and hybridization with Cy3-labeled aptamer DNAs.
2：Sample Selection and Data Sources:
Milk samples were obtained from an online supermarket (Mengniu brand). KANA stock solutions were prepared and diluted for testing.
3：List of Experimental Equipment and Materials:
Chemicals included gold(III) chloride trihydrate, silver nitrate, etc., from various suppliers. Instruments included a laser confocal Raman microscope system (LabRAM HR), spectrophotometer (UV-1800), transmission electron microscope (JEM-1400 Plus and JEM-2100), and potentiometric analyzer (Zetasizer Nano).
4：Experimental Procedures and Operational Workflow:
Synthesis of Au NPs and modification with probe DNA, synthesis of Au@Ag NPs-ssDNA and modification with aptamer DNA, analysis of KANA in milk samples with preprocessing to remove proteins and fats, followed by SERS measurement.
5：Data Analysis Methods:
Raman spectra were collected and analyzed, with detection limit calculated using LOD = 3Sb/b, and selectivity assessed using R ratio.
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Spectrophotometer
UV-1800
Shimadzu Co.
Used to obtain UV-Vis spectra of nanoparticles.
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Transmission Electron Microscope
JEM-1400 Plus
JEOL Ltd.
Used to record TEM images.
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Transmission Electron Microscope
JEM-2100
JEOL Ltd.
Used to obtain HRTEM and HAADF-STEM-EDS mapping.
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Potentiometric Analyzer
Zetasizer Nano
Malvern Instruments Ltd.
Used to acquire zeta potential.
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Laser Confocal Raman Microscope System
LabRAM HR
Horiba France SAS
Used to obtain Raman spectra during KANA detection.
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Milli-Q Water Purification System
Millipore Co.
Used to obtain ultrapure water for experiments.
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