研究目的
To test a new method using the oxidation of cysteamine to quantify the oxygen transmissibility of commercial contact lenses and contact lenses loaded with vitamin E.
研究成果
The methods developed reliably measure oxygen transmissibility of contact lenses, with results within 10% of commercial values for most lenses. Vitamin E loading at 20% does not significantly affect oxygen transmissibility. Further design improvements could enhance accuracy and reliability, making this approach useful for quantifying oxygen transport in various materials.
研究不足
Potential sources of error include air intake during measurements (especially in the eye drop method), lack of controlled mixing in the cuvette method, variations in lens thickness and exposed area, and possible leaks or residual oxygen. Improvements suggested include better sealing, using septa for sampling, adding mixing systems, and designing specialized cuvettes.
1：Experimental Design and Method Selection:
Two custom systems were designed using 3D-printed parts: an eye drop bottle method and a cuvette method. Both systems isolate the contact lens as the only path for oxygen entry into a cysteamine solution, and UV-vis spectrophotometry measures the oxidation rate to calculate oxygen transmissibility based on stoichiometry.
2：Sample Selection and Data Sources:
Commercial contact lenses (e.g., Acuvue Moist, Acuvue TruEye, Acuvue Oasys, Dailies AquaComfort Plus, Dailies Total1, Coopervision Avaira) were used. Cysteamine solutions were prepared in phosphate-buffered saline (PBS) purged with nitrogen to remove oxygen.
3：List of Experimental Equipment and Materials:
Equipment includes a Creality CR-10 3D printer, Monoprice Select 3D Printing Pen, Thermospectronic Genysys 10S UV-Vis spectrophotometer, hole-puncher, quartz cuvettes, eye drop bottles, aluminum foil, super glue, and various chemicals (cysteamine, ethanol, PBS, vitamin E). Materials include PLA filament, contact lenses from specific brands.
4：Experimental Procedures and Operational Workflow:
For the eye drop bottle method, a hole is punched in the bottle, covered with a contact lens using a 3D-printed cap, filled with cysteamine solution, and drops are eluted for measurement. For the cuvette method, a modified cuvette lid holds the contact lens, filled with cysteamine solution, and absorbance is measured directly over time. Both methods involve purging with nitrogen to remove oxygen initially.
5：Data Analysis Methods:
The rate of cysteamine oxidation is measured from UV-vis absorbance data, and oxygen transmissibility is calculated using a stoichiometric equation relating the oxidation rate to oxygen flux through the lens.
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3D printer
CR-10
Creality
Used to create custom 3D-printed components for the experimental setups, such as caps and lids for eye drop bottles and cuvettes.
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3D printing pen
Select
Monoprice
Used for additional 3D printing, such as adding seals to cuvette lids.
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UV-Vis spectrophotometer
Genysys 10S
Thermospectronic
Used to measure the absorbance of cysteamine solutions to determine oxidation rates and calculate oxygen transmissibility.
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Contact lens
Acuvue Moist (Etafilcon A)
Johnson and Johnson Vision Care
Used as the membrane through which oxygen diffuses in the experiments to measure oxygen transmissibility.
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Contact lens
Acuvue TruEye (Narafilcon A)
Johnson and Johnson Vision Care
Used as the membrane through which oxygen diffuses in the experiments to measure oxygen transmissibility.
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Contact lens
Acuvue Oasys (Senofilcon A)
Johnson and Johnson Vision Care
Used as the membrane through which oxygen diffuses in the experiments to measure oxygen transmissibility.
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Contact lens
Dailies Aqua Comfort Plus (Nelfilcon A)
Alcon
Used as the membrane through which oxygen diffuses in the experiments to measure oxygen transmissibility.
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Contact lens
Dailies Total 1 (Delefilcon A)
Alcon
Used as the membrane through which oxygen diffuses in the experiments to measure oxygen transmissibility.
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Contact lens
Avaira (Enfilcon A)
CooperVision
Used as the membrane through which oxygen diffuses in the experiments to measure oxygen transmissibility.
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Eye drop bottle
United States Plastic Corp
Modified to hold the contact lens and cysteamine solution for the eye drop method of measuring oxygen transmissibility.
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Quartz cuvette
Used in the cuvette method to hold the cysteamine solution and allow direct measurement of absorbance through the contact lens.
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