1：Experimental Design and Method Selection:

The study involved synthesizing light-responsive polycarbonates (LrPC and LrPC-PEG) via ring-opening polymerization, blending them with PLGA to form nanoparticle matrices, and preparing nanoparticles using solvent displacement and emulsion diffusion techniques to achieve sizes of about 100 nm and 200 nm. Methods included physicochemical characterization, degradation studies under UV light, drug release kinetics, and cytotoxicity assessments.

2：Sample Selection and Data Sources:

Nanoparticles were prepared with and without the photosensitizer mTHPC. Cell culture studies used HT-29-MTX E-12 cells. Data were derived from laboratory experiments and measurements.

3：List of Experimental Equipment and Materials:

Polymers (PLGA, PEG-PLA, LrPC, LrPC-PEG), photosensitizer mTHPC, solvents (acetone, dichloromethane, ethyl acetate), stabilizers (PVA), cell culture reagents (DMEM, FBS, etc.), and various chemicals from suppliers like Evonik Industries, Sigma-Aldrich, Carl Roth.

4：Experimental Procedures and Operational Workflow:

Nanoparticle preparation involved dissolving polymers and drugs in organic solvents, injecting into aqueous solutions, stirring for solvent evaporation, and purification via centrifugation. Characterization included PCS for size and zeta potential, HPLC for drug quantification, AFM for morphology, and WST-1 assay for cytotoxicity. Illumination was done with a UV LED at 365 nm for 5 min.

5：Data Analysis Methods:

Data were analyzed using one-way ANOVA for statistical significance, with software like Sigma Plot. Measurements were performed in triplicate, and results are presented as mean ± standard deviation.