研究目的
To develop a sensitive and convenient fluorescence-based immunoassay for the detection of imidacloprid pesticide residues, overcoming the limitations of traditional methods such as poor sensitivity and complex synthesis procedures.
研究成果
The developed FIA protocol using CoOOH-AuNCs composite is highly sensitive and selective for imidacloprid detection, with a detection limit of 0.1 ng mL?1 and 60-fold improvement over conventional ELISA. It offers a simplified procedure, reduced toxicity, and potential applications in environmental monitoring and food safety. Future research should explore broader applications and further validation in diverse samples.
研究不足
The study may have limitations in terms of the specificity towards other pesticides or complex matrices, potential interference from high concentrations of certain substances, and the need for optimization of reaction conditions. Future work could focus on expanding the assay to other analytes and improving robustness in real-world samples.
1：Experimental Design and Method Selection:
The study designed a fluorescence-based immunoassay (FIA) using a CoOOH-AuNCs nanocomposite. The rationale was to utilize F?rster resonance energy transfer (FRET) for fluorescence quenching and enzymatic reactions for signal amplification. Methods included synthesis of nanomaterials, fluorescence spectroscopy, and immunoassay procedures.
2：Sample Selection and Data Sources:
Real samples included tap water, river water, rice, and apple, spiked with imidacloprid standards. Data were acquired through fluorescence measurements and compared with gas chromatography.
3：List of Experimental Equipment and Materials:
Equipment included RF-5301 PC spectrofluorophotometer, Shimadzu UV-1700 UV–vis spectrophotometer, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectra. Materials included HAuCl4? xH2O, L-ascorbic acid-2-phosphate (AAP), CoCl2·6H2O, L-glutathione (GSH), ascorbic acid (AA), NaClO, KOH, BSA, trypsin, HRP, GoX, imidacloprid, anti-imidacloprid antibody.
4：Experimental Procedures and Operational Workflow:
Synthesis of CoOOH nanoflakes and AuNCs, formation of CoOOH-AuNCs composite, optimization of conditions for ALP sensing, competitive immunoassay in 96-well plates with coating antigen, antibody binding, addition of ALP-labeled secondary antibody, incubation with AAP and CoOOH-AuNCs, fluorescence measurement.
5：Data Analysis Methods:
Fluorescence intensity measurements, calculation of inhibition efficiency (IE%), linear regression for calibration curves, statistical analysis of recoveries and relative standard deviations (RSDs).
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