研究目的
To develop chitosan-based hydrogels with improved hydrophilic nature and mechanical properties for wound dressing applications, incorporating epidermal growth factor to enhance wound healing.
研究成果
Chitosan-PAA-pHEMA hydrogels exhibit improved hydrophilic and mechanical properties, retain antibacterial and thrombogenic abilities, and when incorporated with EGF, significantly enhance wound healing rates in vivo compared to controls and commercial dressings, making them promising for wound dressing applications.
研究不足
The study may have limitations in scalability for industrial production, potential variability in animal models, and the need for further optimization of hydrogel composition for different wound types. Toxicity of unreacted monomers was minimized but not fully eliminated.
1：Experimental Design and Method Selection:
The study used UV photopolymerization to graft poly(acrylic acid) and poly(hydroxyethyl methacrylate) onto chitosan, aiming to enhance hydrophilicity and mechanical strength. Methods included FTIR analysis, swelling tests, mechanical tests, SEM observation, WVTR measurement, antibacterial assessment, blood clotting test, EGF release studies, and in vivo animal experiments.
2：Sample Selection and Data Sources:
Hydrogel samples were prepared with varying compositions of chitosan, AAc, and HEMA as listed in Table 1. Bacteria strains (Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus) were obtained from Bioresource Collection and Research Center, Taiwan. EGF was sourced from ProSpec, USA. Animal studies used Sprague Dawley rats.
3：Bacteria strains (Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus) were obtained from Bioresource Collection and Research Center, Taiwan. EGF was sourced from ProSpec, USA. Animal studies used Sprague Dawley rats. List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: Homogenizer (Poly Tron; Kinematica, AG, Switzerland), FTIR spectroscopy (Spectrum One, Perkin Elmer, Waltham, MA, USA), universal material tester (AG-IS, Shimadzu, Japan), SEM (S-3000 N, Hitachi, Japan), lyophilizer (FD-5 N, EYELA, Japan), UV absorbance measurement (Lambda 25, Perkin Elmer), ELISA reader (Anthos 2001, Anthos Labtec Instruments, GmbH, Eugendorf, Austria). Materials included chitosan, AAc, HEMA, NMBA, 2-ketoglutaric acid, EGF, and other chemicals of analytical grade.
4：Experimental Procedures and Operational Workflow:
Hydrogels were prepared by mixing components, homogenizing, injecting between glass plates, UV polymerizing, rinsing, and drying. Tests involved swelling in PBS, mechanical tensile tests, SEM for morphology, WVTR measurement using a cup method, antibacterial tests by measuring optical density, blood clotting with rabbit blood, EGF release studies via ELISA, and in vivo wound healing experiments on rats with dressings applied and wound area measured over time.
5：Data Analysis Methods:
Data were analyzed using Student's t-test for statistical significance (p < 0.05), with results reported as means ± SD. Equations were used for calculations such as degree of swelling, water content, WVTR, thrombus rate, and wound closure percentage.
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FTIR Spectroscopy
Spectrum One
Perkin Elmer
Used to analyze functional groups in the hydrogels through infrared spectroscopy.
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Universal Material Tester
AG-IS
Shimadzu
Used to perform tensile tests on hydrogels to measure mechanical properties.
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SEM
S-3000 N
Hitachi
Used to observe the surface and interior morphology of hydrogels.
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UV Absorbance Measurement
Lambda 25
Perkin Elmer
Used to measure optical density for antibacterial assessment.
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Homogenizer
Poly Tron
Kinematica
Used to stir and dissolve components in the hydrogel preparation process.
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Lyophilizer
FD-5 N
EYELA
Used to freeze-dry hydrogel samples for SEM observation.
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ELISA Reader
Anthos 2001
Anthos Labtec Instruments
Used to measure absorbance for EGF release studies.
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