研究目的
To review findings on the SiO2 functionalization process for immobilizing various biomolecules and characterize the surfaces using microelectronic industry techniques to ensure biomolecule activity preservation.
研究成果
SiO2 functionalization using APTES and GOPS protocols effectively immobilizes biomolecules while preserving their activity, as confirmed by TEM, XPS, and bioactivity tests. This supports the use of SiO2 in biosensing applications, enabling miniaturization and integration for smart devices.
研究不足
The study focuses on specific biomolecules and protocols; other immobilization methods or biomolecules may not be covered. Techniques like TEM may cause deterioration in non-covalently immobilized samples. Industrial scalability and real-world application testing are not extensively discussed.
1：Experimental Design and Method Selection:
The study uses APTES and GOPS protocols for covalent immobilization of biomolecules on SiO2 surfaces, involving surface cleaning, silanization, linker attachment, and biomolecule anchoring. Techniques include TEM and XPS for characterization, and spectrophotometric/optical analysis for bioactivity testing.
2：Sample Selection and Data Sources:
Bulk SiO2 surfaces prepared by thermal growth on silicon wafers, cut into slides. Biomolecules include glucose oxidase, horseradish peroxide, metallothionein, and ssDNA.
3：List of Experimental Equipment and Materials:
Transmission Electron Microscopes (Jeol Jem 2010 F and Jeol Jem 2010), XPS spectrometer (Kratos AXIS-HS), spectrophotometric glucose assay kit (Megazyme), optical reader (In-check? by STMicroelectronics), silanes (APTES, GOPS), glutaraldehyde, biomolecules, and various chemicals for surface preparation.
4：Experimental Procedures and Operational Workflow:
Surface cleaning and activation, silanization with APTES or GOPS, biomolecule immobilization, washing, and storage. Characterization via TEM, XPS, and bioactivity tests (e.g., spectrophotometry for GOx activity, XPS for MT metal binding, optical reading for DNA hybridization).
5：Data Analysis Methods:
XPS spectra analysis for elemental composition, TEM imaging and mapping, spectrophotometric measurements at 510 nm, fluorescence intensity analysis for DNA microarrays.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
