研究目的
To analyze the reproducibility of macular and peripapillary thickness measurements, and optic nerve morphometric data obtained with Triton Optical coherence tomography (OCT) in a healthy population.
研究成果
DRI Triton OCT demonstrates good reproducibility for retinal parameters, with macular measurements having the highest reliability. Peripapillary RNFL measurements, particularly in the inferotemporal sector, have lower reliability and should be evaluated with caution. Future improvements should include scans focusing on the nasal quadrant for better assessment in neurodegenerative diseases.
研究不足
Automated perimetry was not performed, which might have allowed inclusion of eyes with subclinical retinal or optic nerve damage. The wide protocol's focus on the fovea may affect reproducibility in peripheral areas like the optic disc. Peripapillary RNFL measurements, especially in inferotemporal sectors, showed lower reliability and should be used cautiously. The study did not compare Triton OCT with other devices, limiting generalizability.
1：Experimental Design and Method Selection:
Observational cross-sectional study using the Triton Swept Source-OCT device with a wide protocol (3D Macular + 5 LineCross) to perform scans. Measurements were repeated three times per eye with random disruption of subject position and focus between acquisitions.
2：Sample Selection and Data Sources:
108 eyes from 108 healthy subjects (age 18-80 years, male/female ratio 52:56) were included. Exclusion criteria: significant refractive errors (>5 diopters spherical equivalent or >3 diopters astigmatism), intraocular pressure ≥21 mmHg, media opacifications, ocular diseases, systemic conditions affecting vision, cup/disc area ratio >
3：All underwent ophthalmologic evaluation including visual acuity, intraocular pressure, and fundoscopic examination. List of Experimental Equipment and Materials:
Triton Swept Source-OCT device (Topcon), using Deep Range Imaging (DRI) technology with a 1050 nm wavelength, scanning speed of 100,000 A-scans per second, axial resolution 8 μm, transverse resolution 20 μm. Software: Microsoft Excel for data registration, SPSS version
4：0 for statistical analysis. Experimental Procedures and Operational Workflow:
Scans performed by an experienced operator using the 3D(H) Macula + 5 LineCross protocol, which provides a
5：0 x 0 mm 3D scan and radial scans for macular and peripapillary areas. Each eye scanned three times with quality score >55 required. Data on macular ETDRS areas, GCL thickness, RNFL thickness, and optic disc morphometry collected. Data Analysis Methods:
Normality tested with Kolmogorov-Smirnov test. Coefficient of variation (COV) calculated as standard deviation/mean and as square root of residual mean squared/mean, both expressed as percentages. Intra-class correlation coefficients (ICC) for absolute agreement calculated. COV <10% considered high reproducibility, <5% very high; ICC interpreted as slight (0-0.2), fair (0.21-0.4), moderate (0.41-0.6), substantial (0.61-0.8), almost perfect (>0.8).
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