1：Experimental Design and Method Selection:

Synthesis of ligands L1 and L2 via condensation reactions, followed by synthesis of zinc complexes R1 and R2. Characterization using spectroscopic methods (NMR, FTIR, mass spectrometry), X-ray crystallography, and photophysical studies. Chemosensor studies with anions in aqueous HEPES buffer. Cell imaging with HeLa cells and cytotoxicity assays. Alkaline phosphatase activity assays.

2：Characterization using spectroscopic methods (NMR, FTIR, mass spectrometry), X-ray crystallography, and photophysical studies. Chemosensor studies with anions in aqueous HEPES buffer. Cell imaging with HeLa cells and cytotoxicity assays. Alkaline phosphatase activity assays. Sample Selection and Data Sources:

2. Sample Selection and Data Sources: Ligands synthesized from o-phenylenediamine and 4-pyridinecarboxaldehyde. Zinc complexes prepared with ZnCl2 and Zn(NO3)2. Anions and enzymes sourced from Sigma Aldrich and other suppliers. HeLa and MCF-7 cell lines from National Centre for Cell Sciences.

3：Anions and enzymes sourced from Sigma Aldrich and other suppliers. HeLa and MCF-7 cell lines from National Centre for Cell Sciences. List of Experimental Equipment and Materials:

3. List of Experimental Equipment and Materials: JEOL NMR spectrometer (400 MHz), Bruker Tensor 27 FTIR spectrophotometer, Fisons CHN analyzer, PAN analytical X'PERT PRO diffractometer, Mettler Toledo TGA, Shimadzu UV-2600 spectrophotometer, Perkin L55 fluorometer, Metrohm Microtrac Ultra Nanotrac DLS, Hitachi H-7500 TEM, Bruker AFM, Pico Quant FluoTime 300 lifetime spectrometer, Carl Zeiss fluorescence microscope, Tecan M200 PRO microplate reader.

4：Experimental Procedures and Operational Workflow:

Ligand synthesis by stirring reactants, complex synthesis by mixing solutions and crystallization. Photophysical measurements in quartz cuvettes. Anion binding studies by adding analytes to complex solutions. Cell culture and imaging with incubation and fixation. ALP activity monitored by fluorescence intensity changes over time.

5：Data Analysis Methods:

HyperSpec software for stability constants, GraphPad Prism for kinetic parameters, IUPAC 3σ method for detection limits, bi-exponential fitting for lifetime data.