研究目的
To assess the relationship between combined serum lutein and zeaxanthin (L+Z) concentration and macular pigment optical density (MPOD), and to investigate the effect of L+Z+docosahexaenoic acid (DHA) dietary supplementation on the spatial distribution of MPOD.
研究成果
Juxtafoveal MPOD was associated with serum L+Z concentration, and foveal MPOD increased with L+Z+DHA supplementation. Contrast sensitivity improved after supplementation. The study provides preliminary data supporting the relationship and effects, but further research is needed to isolate individual component effects and use more specific measurement devices.
研究不足
The effects of L, Z, and DHA on changes in MPOD and CS were not identified separately. The HPLC system could only measure combined L+Z serum concentration, not separately. The study had a relatively small number of participants, and there was an outlier in serum L+Z data at baseline.
1：Experimental Design and Method Selection:
The study was designed as a preliminary analysis using a dietary supplement intervention. MPOD was estimated using fundus autofluorescence (FAF) images with a one-wavelength method due to instrument constraints. Statistical analyses included paired t-tests, Wilcoxon signed-rank tests, and Pearson correlation coefficients.
2：Sample Selection and Data Sources:
Twenty healthy fellow eyes from patients with unilateral wet age-related macular degeneration or chronic central serous chorioretinopathy were selected. Exclusion criteria included other ocular diseases, intraocular surgery, serious systemic diseases, recent supplement use, and inability to provide informed consent.
3：List of Experimental Equipment and Materials:
Confocal scanning laser ophthalmoscope (Heidelberg Retinal Angiograph, HRA2; Heidelberg Engineering), MATLAB R2009a software (MathWorks), CSV-1000E Contrast Testing Instrument (Vector Vision), serum separator tubes, high-performance liquid chromatography (HPLC) system, and dietary supplement Sante Lutax 20 plus DHA (Santen Pharmaceutical).
4：Experimental Procedures and Operational Workflow:
Participants received a daily supplement for 6 months. Measurements of best-corrected visual acuity, contrast sensitivity, and MPOD were taken at baseline, 1, 3, and 6 months. Serum L+Z concentrations were measured at baseline and 3 months using HPLC. FAF images were processed using MATLAB for MPOD calculation.
5：Data Analysis Methods:
Data were analyzed using paired t-tests for MPOD comparisons, Wilcoxon signed-rank tests for non-parametric CS data, and Pearson correlation for serum L+Z and MPOD relationships.
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