研究目的
To evaluate the biomechanical stability of the corneal scar treating with riboflavin and ultraviolet A (UVA).
研究成果
CXL with riboflavin/UVA applied seven days after suturing significantly improved the biomechanical properties of corneal scars, as evidenced by increased ultimate stress and Young's modulus, and higher α-SMA expression. This suggests that delayed CXL enhances corneal strength and could be a potential therapy for preventing complications like corneal staphyloma, but long-term studies are required.
研究不足
The study was conducted on rabbits, which may not fully replicate human corneal responses. The observation period was limited to 8 weeks, so long-term effects are unknown. The lesion size was larger than in some previous studies, potentially affecting recovery times. Further research is needed to confirm the mechanisms and applicability to humans.
1：Experimental Design and Method Selection:
The study used an animal model with New Zealand rabbits to create corneal penetrating injuries and applied riboflavin/UVA-induced collagen cross-linking (CXL) at different time points post-suturing. Groups were divided into control and trauma groups with sub-groups based on sacrifice times (4, 6, and 8 weeks). Biomechanical properties (stress and Young's modulus) and α-SMA expression were evaluated.
2：Sample Selection and Data Sources:
86 healthy rabbits were used, with right corneas penetrated using blades. Samples included corneal scar strips and tissues for Western blot analysis.
3：List of Experimental Equipment and Materials:
Equipment included a UVA diodes system (Zhuhai Tianhui Electronic Co.), UV light meter (UV-340A), stress-strain tester (CMT6104), digimatic calliper (Sanling group), and materials such as riboflavin solution (Beijing Solarbio Science & Technology Co., Ltd.), sodium pentobarbital (Beijing Pubos Biotechnology Co., Ltd.), antibodies (Beijing Boorsen Biotechnology Co., Ltd.), and sutures.
4：Experimental Procedures and Operational Workflow:
Rabbits were anesthetized, corneas were penetrated and sutured. CXL was applied immediately or after 7 days using riboflavin and UVA irradiation. At sacrifice times, corneal strips were cut and subjected to stress-strain tests, and tissues were analyzed for α-SMA expression via Western blot.
5：Data Analysis Methods:
Data were analyzed using one-way ANOVA with SPSS software (version 17.0), with statistical significance set at P<0.05.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容-
sodium pentobarbital solution
2%
Beijing Pubos Biotechnology Co., Ltd.
Used for anesthesia in rabbits during corneal injury procedure.
-
riboflavin photosensitizer solution
0.1%
Beijing Solarbio Science & Technology Co., Ltd.
Applied to the cornea to induce collagen cross-linking with UVA irradiation.
-
UVA diodes
Zhuhai Tianhui Electronic Co.
Used for UVA irradiation at 370 nm wavelength to activate riboflavin for cross-linking.
-
UV light meter
UV-340A
Monitored the illumination intensity during UVA irradiation.
-
digimatic calliper
Sanling group (H.K.) Ltd.
Measured the thickness of corneal scar strips.
-
stress-strain tester
CMT6104
Meitesi industry system Co. Ltd.
Performed stress-strain tests on corneal strips to evaluate biomechanical properties.
-
primary antibodies
Beijing Boorsen Biotechnology Co., Ltd.
Used in Western blot analysis to detect α-SMA expression.
-
secondary antibody
BoAoSeng
Horseradish peroxidase-conjugated secondary antibody for Western blot.
-
登录查看剩余6件设备及参数对照表
查看全部
