研究目的
To develop a multifunctional nanoagent for CT/MRI-guided synergistic radiotherapy and X-ray inducible photodynamic therapy of tumors, aiming to overcome the limitations of traditional photodynamic therapy by using X-rays for deeper tissue penetration and enhanced therapeutic effects.
研究成果
The GWOT@MC540 NPs effectively enable synergistic RT&X-PDT with dual-modal CT/MRI imaging, showing higher tumor growth inhibition at lower X-ray doses compared to radiotherapy alone. The nanoagents exhibit low cytotoxicity and are safely cleared from the body, indicating potential for clinical translation in cancer theranostics.
研究不足
The study is a proof-of-concept; further investigations are needed for optimization, such as using clinical megavolt photon beams, reducing required nanoagent concentrations, and exploring X-ray luminescence-mediated imaging. The in vivo studies were limited to mouse models, and long-term effects require more extensive evaluation.
1：Experimental Design and Method Selection:
The study involved synthesizing Gd2(WO4)3:Tb nanoparticles (GWOT NPs) via a hydrothermal method, coupling them with Merocyanine 540 (MC540) for X-ray inducible photodynamic therapy (X-PDT), and evaluating their dual-modal imaging (CT and MRI) and synergistic therapeutic capabilities. Theoretical models included radioluminescence and photoluminescence spectroscopy for energy transfer analysis.
2：Sample Selection and Data Sources:
Samples included GWOT NPs, GWOT-PEG NPs, and GWOT@MC540 NPs. In vitro studies used murine breast cancer cells (4T1) and human bronchial epithelial cells (Beas-2B). In vivo studies involved 4T1 tumor-bearing mice.
3：List of Experimental Equipment and Materials:
Equipment included TEM for imaging, XRD for crystallography, DLS for size analysis, RS-2000 Pro biological system for X-ray irradiation, CCK-8 assay for cytotoxicity, flow cytometry for apoptosis analysis, CT and MRI scanners for imaging. Materials included cetyltrimethylammonium bromide, PEG 600, MC540, SOSG indicator.
4：Experimental Procedures and Operational Workflow:
Synthesis of GWOT NPs, PEG coating, MC540 loading, characterization (TEM, XRD, DLS), in vitro assays (cytotoxicity, 1O2 production, cell viability, apoptosis), in vivo studies (tumor growth inhibition, imaging via CT and MRI, biodistribution, toxicity assessment).
5：Data Analysis Methods:
Statistical analysis using p-values, linear regression for relaxation rates, comparison of HU values for CT, and cell viability assays.
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