研究目的
To describe MNRF-induced micropores using RCM and OCT imaging.
研究成果
RCM and OCT are complementary in vivo imaging modalities for assessing MNRF micropores, with distinct morphological differences based on RF energy. RCM provides visualization of shape, content, and coagulation zones, while OCT assesses depth and number of micropores. High RF energy results in more detectable and deeper micropores with coagulation extending to deeper skin layers, whereas low RF energy causes superficial changes. These findings support the use of combined imaging for evaluating MNRF treatments.
研究不足
The study has a small number of participants. Gender and age differences may affect micropore characteristics, and only two RF energy levels were used, limiting the assessment range. Histology did not show demarcated loss of substance, and some OCT features might be artifacts.
1：Experimental Design and Method Selection:
The study used an intra- and inter-individual approach to compare high and low RF energy MNRF treatments. RCM and OCT were employed for in vivo imaging to characterize micropore morphology, with histology used for correlation.
2：Sample Selection and Data Sources:
Five healthy participants (three women and two men, age 22-54) were recruited from the Department of Dermatology at Bispebjerg Hospital. Treatment areas were on the lateral right hip, shaved to remove hairs.
3：List of Experimental Equipment and Materials:
A 7x7 array of 49 insulated gold-coated microneedles (Lutronic Infini?, Lutronic Aesthetic, Burlington, MA, USA) with a fixed needle length of 1500 μm was used. RCM imaging was performed with a Vivascope Multilaser 1500? (Caliber ID Inc., Rochester, NY, USA). OCT imaging used a VivoSight Dx (Michelson Diagnostics, Kent, UK). Histology involved punch biopsies sectioned at 10 μm thickness and stained with hematoxylin and eosin. Fiji ImageJ software and SPSS software (version
4：0) were used for analysis. Experimental Procedures and Operational Workflow:
Participants received MNRF treatment with high RF energy (conduction time 500 msec,
5：5 W, energy level 3) or low RF energy (conduction time 10 msec, 5 W, energy level 1) on adjacent areas. RCM imaging was performed immediately after treatment, acquiring VivaBlocks and VivaStacks. OCT scanning followed with multi-slice modality. One participant underwent punch biopsies for histology. Data Analysis Methods:
RCM images were analyzed for shape, content, and coagulation zone thickness using Fiji ImageJ. OCT images were analyzed for micropore count and depth using integrated software. Descriptive statistics (medians and IQR) were calculated using SPSS due to non-normal distribution.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
