研究目的
To demonstrate the feasibility of using Fourier transform IR spectroscopy of saliva for the diagnosis of lung and breast cancer.
研究成果
Feasibility in principle has been shown for diagnosing lung and breast cancer using Fourier transform IR spectroscopy of saliva. Statistically significant differences were observed in specific spectral ranges and intensity ratios (I1398/1454 and I1240/1310). The greatest differences for breast cancer were in early stages, and for lung cancer in advanced stages, suggesting potential for early diagnosis of breast cancer. Future work should focus on developing classifying systems and evaluating diagnostic sensitivity and specificity.
研究不足
The study is limited by the inclusion of only two types of cancer (lung and breast), a small patient sample size, and the heterogeneity of the lung cancer group with different histological types. This hinders drawing broad conclusions and requires further research with larger samples and more cancer types. The correlations found with nonspecific biochemical parameters may limit clinical utility.
1：Experimental Design and Method Selection:
The study used Fourier transform IR spectroscopy to analyze saliva samples from cancer patients and healthy controls. Statistical analysis was performed using nonparametric methods with the StatSoft Statistica
2：0 program, including the Wilkinson criterion for dependent groups and the Mann-Whitney U-criterion for independent groups. Sample Selection and Data Sources:
Two groups were studied: a major group of 40 lung cancer patients (men) and 50 breast cancer patients (women), and a control group of 55 men and 58 women without cancer pathology. Selection criteria included age 30-75 years, no current treatment, no active infection, and histological verification of diagnosis. Saliva samples (1 mL) were collected from fasting patients in the morning.
3：List of Experimental Equipment and Materials:
A Simex FT801 Fourier transform IR spectrometer was used to take IR absorption spectra at 500-4000 cm–
4：Saliva samples were centrifuged at 7000 rpm and dried on a zinc selenide plate at 37°C. Experimental Procedures and Operational Workflow:
Saliva was collected, centrifuged, dried, and then IR spectra were measured. Data were analyzed statistically with significance set at p <
5：Data Analysis Methods:
Band intensities and ratios were compared between groups using statistical tests, and discriminant analysis was applied for multidimensional evaluation.
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