1：Experimental Design and Method Selection:

The study employs a signal amplification strategy where glucose oxidase catalyzes glucose oxidation to produce H2O2, which oxidizes silver nanocubes into Ag+ ions. These Ag+ ions then enhance the fluorescence of a specific probe (Ag+-FP). The methodology includes synthesis of AgNC-GOx conjugate and Ag+-FP, followed by in vitro and cellular assays for glucose detection.

2：Sample Selection and Data Sources:

Aqueous solutions with varying glucose concentrations and five different cell lines (MCF-10a, HeLa, MCF-7, A375, 4T1) were used. Cells were cultured in DMEM with 10% FBS and 1% penicillin/streptomycin.

3：List of Experimental Equipment and Materials:

Materials include ethylene glycol, silver trifluoroacetate, thioctic acid, glucose oxidase, poly(vinyl pyrrolidone), Rhodamine B, various sugars, H2O2 Assay Kit, and others from suppliers like JK Chemical, Sigma-Aldrich, MCE. Equipment includes Synergy H1 microplate reader, Thermo Scientific Lumina fluorescence spectrophotometer, Cary 60 UV-vis spectrophotometer, JEM-1230 TEM, Malven Zetasizer 2000, FTIR spectrometer, IVIS Spectrum system.

4：Experimental Procedures and Operational Workflow:

Synthesis of AgNC-GOx involves conjugating thioctic acid to AgNC via Ag-S bond, then conjugating with GOx using NHS and EDC. Ag+-FP is synthesized from Rhodamine B. For glucose detection, AgNC-GOx is incubated with glucose solutions or cells, followed by addition of Ag+-FP and fluorescence measurement.

5：Data Analysis Methods:

Fluorescence intensity is measured and correlated with glucose concentration. Statistical analysis includes mean ± SD and Student's t-test for cellular experiments.