1：Experimental Design and Method Selection:

The study uses hollow gold nanoshells (HGNs) as a delivery vehicle for the NuBCP-9 peptide, with light-activated release via near-infrared (NIR) irradiation. Orthogonal surface chemistry is employed for peptide loading and internalization.

2：Sample Selection and Data Sources:

Cancer cell lines including HeLa, MDA-MB-231, and H460 paclitaxel-resistant (PacR) cells are used. Zebrafish embryos serve as an in vivo model for xenograft studies.

3：List of Experimental Equipment and Materials:

HGNs synthesized via galvanic replacement, peptides (NuBCP-9, TAT), DNA strands, streptavidin, dyes (Cy5, FAM, JC-1), lasers (Ti:sapphire femtosecond pulsed laser), microscopes (Olympus BX51, Zeiss LSM 780 confocal), plate reader (Tecan M200).

4：0). Experimental Procedures and Operational Workflow:

4. Experimental Procedures and Operational Workflow: HGN synthesis, surface modification with DNA and peptides, cellular internalization studies, laser irradiation for peptide release, viability assays (PrestoBlue), mitochondrial depolarization assays (JC-1), and in vivo zebrafish xenograft experiments with imaging and laser treatment.

5：Data Analysis Methods:

Fluorescence quantification using calibration curves, image analysis with Fiji (ImageJ) software, statistical analysis including two-way ANOVA with Sidak's multiple comparisons test.