1：Experimental Design and Method Selection:

The study used genetic mouse models (Opn5-null, conditional deletions) and lighting conditions (full spectrum VBGR vs. minus violet BGR) to assess hyaloid vessel regression. Methods included immunohistochemistry, ELISA for dopamine quantification, immunoblotting for protein analysis, and pharmacological interventions (e.g., DAT inhibitor GBR12909).

2：9). Sample Selection and Data Sources:

2. Sample Selection and Data Sources: Postnatal mice (P1 to P24) of various genotypes (e.g., Opn5+/+, Opn5?/?, conditional mutants) were used. Samples included retinal tissues, hyaloid vessels, and vitreous fluid. Data were sourced from multiple litters to ensure reproducibility.

3：List of Experimental Equipment and Materials:

Equipment included Zeiss ApoTome AX10 and LSM700 confocal microscopes for imaging, EnVision Multimode Plate Reader for ELISA, and standard laboratory equipment for immunoblotting. Materials included antibodies (e.g., anti-TH, anti-DAT, anti-VEGFR2), pharmacological reagents (e.g., GBR12909, SKF38393), and genetically modified mouse lines.

4：Experimental Procedures and Operational Workflow:

Procedures involved dark adaptation and light exposure (380-nm light), tissue dissection, immunofluorescence staining, ELISA for dopamine levels, immunoblotting for protein phosphorylation, and daily pharmacological injections from P1 to P8. Hyaloid vessels were quantified from labeled preparations.

5：Hyaloid vessels were quantified from labeled preparations. Data Analysis Methods:

5. Data Analysis Methods: Statistical analyses used Student's t-test, one-way or two-way ANOVA as appropriate, with post-hoc tests (Sidak's or Tukey's). Data were analyzed using GraphPad Prism and ImageJ for band intensity quantification.