研究目的
To develop a sensitive and reliable sensor for real-time and non-invasive dissolved oxygen (DO) detection in bioprocess by using a morphology-tuning strategy to improve the sensitivity and responsiveness of DO polymeric sensors.
研究成果
The morphology-tuning strategy effectively improves the sensitivity and responsiveness of DO sensors by enhancing hydrophobicity and forming interconnected porous networks. P(Pt-TPP-TFE-POSS) demonstrated the highest performance with excellent linear Stern-Volmer behavior, stability, and reversibility, enabling real-time, non-invasive DO monitoring in bioprocesses like cephalosporin C fermentation. This approach provides a valuable tool for industrial and biological applications and suggests further optimization of sensor materials for broader use.
研究不足
The study focuses on specific polymers and moieties (Ad and POSS); other morphology-tunable moieties were not explored. The sensor performance was tested in controlled aqueous systems and a specific fermentation process (cephalosporin C), which may not generalize to all biological or industrial environments. Long-term stability under extreme conditions (e.g., high temperatures or varied pH beyond tested range) was not fully assessed. The fabrication process involves multiple steps, which could be optimized for scalability.
1：Experimental Design and Method Selection:
The study employed a morphology-tuning strategy by incorporating different morphology-tunable moieties (adamantane (Ad) and polyhedral oligomeric silsesquioxanes (POSS)) into copolymers with oxygen-sensitive platinum(II) tetraphenylporphyrin (Pt-TPP) and trifluoroethyl methacrylate (TFE) to enhance oxygen permeability and sensor performance. Polymerization was conducted under homogeneous conditions using free radical polymerization with AIBN as initiator.
2：Sample Selection and Data Sources:
Polymers P(Pt-TPP-TFE), P(Pt-TPP-TFE-Ad), and P(Pt-TPP-TFE-POSS) were synthesized and characterized. Sensor films were fabricated by coating polymer solutions on PET substrates. DO measurements were performed in aqueous solutions and during cephalosporin C fermentation.
3：List of Experimental Equipment and Materials:
Equipment included Bruker AVANCE III 400 MHz Spectrometer for NMR, Waters LCT Premier XE for HRMS, Agilent Cary 60 UV-Vis Spectrophotometer, LengGuang Technologies F97Pro fluorescence spectrophotometer, Waters 1515 GPC, POWEREACH JC2000C for water contact angles, HITACHI S3400N SEM, and Edinburgh Instruments FLS920 fluorometer with microsecond Flashlamp μF900H. Materials included platinum(II) chloride, POSS-NH2, trifluoroethyl methacrylate, amantadine hydrochloride, methacryloyl chloride, AIBN, and other chemicals.
4：0H. Materials included platinum(II) chloride, POSS-NH2, trifluoroethyl methacrylate, amantadine hydrochloride, methacryloyl chloride, AIBN, and other chemicals. Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Synthesis involved polymerization of monomers in Schlenk tubes, followed by platinum coordination. Sensor films were prepared by coating and drying. DO sensitivity and response were evaluated by measuring phosphorescence intensity under varying oxygen conditions (N2-, air-, O2-saturated). Real-time monitoring was conducted in a microbioreactor during fermentation.
5：Data Analysis Methods:
Sensitivity was quantified using quenching response (QDO = I0/I100). Stern-Volmer plots were used for quantitative oxygen detection. Phosphorescence lifetimes were analyzed with double-exponential fitting. Data were processed using standard spectroscopic and statistical methods.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容-
Spectrometer
AVANCE III 400 MHz
Bruker
Recording 1H and 13C NMR spectra for characterization of monomers and polymers.
暂无现货
预约到货通知
-
UV-Vis Spectrophotometer
Cary 60
Agilent
Performing absorption spectra measurements for photophysical characterization.
Cary 60 UV-Vis Spectrophotometer
立即获取同款设备
-
Scanning Electron Microscope
S3400N
HITACHI
Recording SEM images to investigate morphology and porosity of sensor films.
暂无现货
预约到货通知
-
Fluorometer
FLS920
Edinburgh Instruments
Acquiring microsecond time-resolved decay time data for phosphorescence lifetime analysis.
立即获取同款设备 -
Mass Spectrometer
LCT Premier XE
Waters
Measuring high-resolution mass spectra (HRMS) for characterization of intermediate monomers.
暂无现货
预约到货通知
-
Fluorescence Spectrophotometer
F97Pro
LengGuang Technologies
Performing fluorescence and phosphorescence spectra measurements for sensor evaluation.
暂无现货
预约到货通知
-
Gel Permeation Chromatography
1515
Waters
Conducting molecular weight and polydispersity analysis of polymers.
暂无现货
预约到货通知
-
Contact Angle Measuring Instrument
JC2000C
POWEREACH
Determining water contact angles to assess hydrophobicity of sensor films.
暂无现货
预约到货通知
-
登录查看剩余6件设备及参数对照表
查看全部
