1：Experimental Design and Method Selection:

The study involved synthesizing nine Pt(II) Schiff base complexes (Pt1-Pt9) with varying substituents to investigate structure-activity relationships. Methods included synthesis, characterization (NMR, UV-Vis, mass spectrometry, elemental analysis, X-ray crystallography), photophysical and photochemical property measurements (phosphorescence spectroscopy, singlet oxygen quantum yield determination, cyclic voltammetry), and biological assays (cell culture, cytotoxicity, cellular imaging).

2：Sample Selection and Data Sources:

Samples included synthesized Pt(II) complexes and ligands, A549 human lung cancer cells from ECACC, and commercial reagents. Data were obtained from instrumental analyses and cell-based assays.

3：List of Experimental Equipment and Materials:

Equipment included Xcalibur Gemini diffractometer, CH Instrument model 600D Electrochemical Analyzer, Bruker DPX 300/400 NMR spectrometers, Cary 300 UV-vis spectrophotometer, JASCO FP-6500 spectrofluorometer, Fluorolog spectrofluorometer, GloMax-Multi Microplate Multimode Reader, LSM 710 confocal microscope. Materials included solvents, buffer components, K2PtCl4, sodium acetate, organelle dyes (MitoTracker, LysoTracker, Hoechst 33342), and cell culture reagents.

4：Experimental Procedures and Operational Workflow:

Synthesis of ligands and complexes via condensation and coordination reactions. Characterization using NMR, UV-Vis, mass spectrometry, elemental analysis, and X-ray crystallography. Photophysical measurements (absorption, emission, quantum yields, lifetimes) in various solvents. Electrochemical studies via cyclic voltammetry. Singlet oxygen generation assays using RNO/imidazole method. Cell culture maintenance, cytotoxicity assays (dark and light conditions), and confocal microscopy for cellular localization.

5：Data Analysis Methods:

Data were processed using software like MestReC, TOPSPIN, UV Winlab, OriginPro 2016. Statistical analysis for IC50 values, and comparison with standards for quantum yields and electrochemical potentials.