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- 摘要
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- 实验方案
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Facile synthesis of novel carbon-dots/hemin nanoplatforms for synergistic photo-thermal and photo-dynamic therapies
摘要： Due to the traditional therapies of cancer inducing huge pains to patients, the non-invasive photo-guided therapies are attracting massive attentions of researchers. Herein, the intelligent-designed carbon-dots/hemin nanoplatforms (HCDs NPs) were developed, owning high-authority photo-therapy for cancer. The fluorescence resonance energy transfer (FRET) effect enhanced the photo-thermal ability of HCDs NPs, endowing the synthesized nanoplatforms with photo-dynamic property simultaneously. Therefore, the obtained HCDs NPs could achieve synergetic photo-thermal and photo-dynamic therapies for cancer. Basing on the experimental results, the prepared HCDs NPs could induce the temperature enhancement high to ca 26 ℃ under laser irradiation, also with the outstanding photo-dynamic efficacy. More than 90 % of cancer cells die after 10 min laser treatment. Thus, the dual-modal photo-therapeutic HCDs NPs are promising and excellent nanomaterials for potential application in synergistic cancer therapy.
关键词： Dual-modal nanoplatform,Synergistic cancer therapy,FRET effect,Enhanced photo-thermal therapy,Photo-dynamic therapy
更新于2025-09-19 17:15:36
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Novel multi-responsive fluorescence switch for Hg2+ and UV/vis lights based on diarylethene-rhodamine derivative
摘要： A new multi-responsive fluorescent switch DTE-Pip-Rho 1O has been designed and synthesized in this study. For this molecule, the reactive site of spirolactam ring is far away from the diarylethene unit through the intramolecular piperazine. It exhibited high selectivity and sensitivity to Hg2+ in addition to obvious color change (colorless-pink) and fluorescence “off-on” (dark-orange) during this process. It is believed that the color change is due to Hg2+-assisted hydrolysis of rhodamine hydrazide. Therefore, the new molecule can be used as colorimetric and fluorescent chemosensor for Hg2+ with high selectivity. The detection limits of absorbance and fluorescence for Hg2+ were calculated to be 1.15 μM and 1.16 μM, respectively. The resulting DTE-Pip-Rho 1O-Hg2+ could also function as a reversible fluorescence photo-switch in response to UV/vis light owing to FRET mechanism. Moreover, it has been demonstrated that the photo-switchable system displayed excellent fatigue resistance and remarkable anti-photobleaching capability.
关键词： Multi-responsive,Fluorescent switch,Diarylethene,FRET mechanism,Sensor,Rhodamine
更新于2025-09-19 17:15:36
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Quantum Dots for F??rster Resonance Energy Transfer (FRET)
摘要： The analysis of biomolecular interactions using quantum dots (QDs) as both FRET donors and acceptors has become an established technique in the life sciences. This development has been driven by the unique properties of QDs, which include large surfaces for the attachment of biomolecules, high brightness and photostability, strong and spectrally broad absorption, and color-tunability via QD size, shape, and material. Applications include molecular rulers for structural analysis, small-molecule sensors, immunoassays, enzyme assays, nucleic acid assays, fluorescence imaging in-vitro and in-vivo, and molecular logic gates. Here, we will explain the theory of QD-based FRET, review some aspects of QD surface functionalization that are important for FRET, and highlight and discuss the advantages and disadvantages of QDs in FRET-biosensing using both spectroscopy and imaging techniques.
关键词： immunoassays,multiplexing,biosensors,FRET,imaging,quantum dots
更新于2025-09-19 17:13:59
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CdSe quantum dots-sensitized FRET system for ciprofloxacin detection
摘要： The prepared CdSe quantum dots (QDs) were applied to selectively and sensitively assay ciprofloxacin as a fluorescence sensor based on fluorescence resonance energy transfer (FRET) mechanism. The efficiency of FRET between CdSe QDs and ciprofloxacin can be modulated by the degree of spectral overlap between the excitation peak of CdSe QDs and the emission peak of ciprofloxacin. Within the range of 0 to 120 μmol·L?1, the good linearity was obtained (R = 0.99561) and the detection limit reached to 0.6 μmol·L?1. This method is simple and rapid and can be used in environmental water and milk samples.
关键词： Fluorescent sensor,Semiconductors,Luminescence,Ciprofloxacin,CdSe quantum dots,FRET
更新于2025-09-19 17:13:59
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Nonradiative Energy Transfer between Thickness-Controlled Halide Perovskite Nanoplatelets
摘要： Despite showing great promise for optoelectronics, the commercialization of halide perovskite nanostructure-based devices is hampered by inefficient electrical excitation and strong exciton binding energies. While transport of excitons in an energy-tailored system via F?rster resonance energy transfer (FRET) could be an efficient alternative, halide ion migration makes the realization of cascaded structures difficult. Here, we show how these could be obtained by exploiting the pronounced quantum confinement effect in two-dimensional CsPbBr3-based nanoplatelets (NPls). In thin films of NPls of two predetermined thicknesses, we observe an enhanced acceptor photoluminescence (PL) emission and a decreased donor PL lifetime. This indicates a FRET-mediated process, benefitted by the structural parameters of the NPls. We determine corresponding transfer rates up to kFRET = 0.99 ns?1 and efficiencies of nearly ηFRET = 70%. We also show FRET to occur between perovskite NPls of other thicknesses. Consequently, this strategy could lead to tailored energy cascade nanostructures for improved optoelectronic devices.
关键词： nanoplatelets,quantum confinement,FRET,halide perovskite,optoelectronics
更新于2025-09-19 17:13:59
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An energy and charge transfer synergetic donora??acceptor heterostructure 2D-COF in photovoltaics
摘要： The formation of orderly p-n heterojunctions by the wafer-scale alignment of donor (D) and acceptor (A) molecules, important to achieve high photocurrent generation in the organic semiconductor-based organization, remains a challenging topic. Presented herein is a distinctive D-A heterostructure two-dimensional organic covalent frameworks (2D-COF) as an efficient organic photovoltaic (OPV) film, supported by the triple roles of the binary building blocks in wafer-scale film growing at water/oil interface, molecular level morphology control, and synergistic F?rest resonance energy transfer (FRET) and charge-transfer (CT) functions. The achieved D-A heterostructure 2D-COF has a wafer-scale size, efficient spectral response, and effective separation of photogenerated electron-hole pairs, resulting in an efficient photocurrent generation which is much larger than those of reported OPV COF materials. Achieved herein confirm that the marriage of FRET and CT synergetic D-A heterostructure and ultrathin 2D-COF film offer unparalleled advantages in OPV.
关键词： Photovoltaics,FRET,Donor-Acceptor heterostructure,Charge-transfer,2D-COF
更新于2025-09-19 17:13:59
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FRET study between Carbon Quantum Dots and Malachite Green by Steady State and Time Resolved Fluorescence Spectroscopy
摘要： Background: Understanding the interaction between different organic dyes and carbon quantum dots helps us to understand several photo physical processes like electron transfer, energy transfer, molecular sensing, drug delivery and dye degradation processes etc. Objective: The primary objective of this study is to whether the carbon quantum dots can act as an electron donor and can participate in the different photo physical processes. Methods: In this work, carbon quantum dots (CQDLs) are synthesized in most economical and simple carbonization method where petals of Nelumbo nucifera L. are used as a carbon precursor. The synthesized CQDLs were characterized by using experimental techniques like UV?Vis absorption, FT-IR, transmission electron microscopy (TEM), steady-state and time-resolved fluorescence spectroscopy. Results: The spectral analysis shows that the so synthesized CQDLs are spherical in shape and its diameter is around 4.2 nm. It shows the fluorescence emission maximum at 495 nm with a quantum yield of 4%. In this work the interaction between carbon quantum dots (CQDLs) and an organic dye malachite green (MG) is studied using fluorescence spectroscopic technique under ambient pH condition (At pH 7). The quenching mechanism of CQDLs with MG was investigated using Stern-Volmer equation and time-resolved fluorescence lifetime studies. The results show that the dominant process of fluorescence quenching is attributed to Forster Resonance Energy Transfer (FRET) having a donor acceptor distance of 53 ? where CQDLs act as a donor and MG acts as an acceptor. Conclusion: This work has a consequence that CQDLs can be used as a donor species for different photo physical processes such as photovoltaic cell, Dye sensitized solar cell, and also for antioxidant activity study.
关键词： Carbon quantum dots,stern-volmer plot,Nelumbo nucifera L.,FRET,malachite green
更新于2025-09-19 17:13:59
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Fluorometric visualization of mucin 1 glycans on cell surfaces based on rolling-mediated cascade amplification and CdTe quantum dots
摘要： A rolling-mediated cascade (RMC) amplification strategy is described for improved visualization of profiling glycans of mucin 1 (MUC 1) on cell surfaces. CdTe quantum dots (QDs) are used as fluorescent labels. The RMC based amplification allows even distinct glycoforms of MUC1 to be visualized on the surface of MCF-7 cell via an amplified F?rster resonance energy transfer (FRET) imaging strategy that works at excitation/emission wavelengths of 345/610 nm. This is achieved by utilizing antibody against MUC1 modified with the fluorescent label 7-amino-4-methylcoumarin-3-acetic acid (AMCA) as the energy donor in FRET. The QDs (used to label surface glycans) act as acceptors. N-Azidoacetylgalactosamine-Acetylated (Ac4GalNAz) as a non-natural azido sugar, can be incorporated into the glycans of the cell surface, which can promote further labeling. The method has the advantage of only requiring a small amount of non-natural sugar to be introduced in metabolic glycan labeling since too much of an artificial sugar will interfere with the physiological functions of cells.
关键词： Cancer marker,Azide polysaccharide,FRET,Glycosylation,Glycoprotein,DNA probe,Quantum dots,Click reaction,Metabolic labeling,Rolling-mediated cascade amplification
更新于2025-09-19 17:13:59
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CsPbBr <sub/>3</sub> Quantum Dots as Artificial Antennas to Enhance the Light-Harvesting Efficiency and Photoresponse of Zinc Porphyrin
摘要： Broadening the spectral range and enhancing the efficiency of light-harvesting materials are important to the design of novel optoelectronic devices. In this work, CsPbBr3 quantum dots (QDs) are introduced as artificial antennas to enhance both the light-harvesting efficiency and photoresponse of zinc porphyrin (ZnP). The Fluorescence Resonance Energy Transfer (FRET) process both in solution and films from the donor CsPbBr3 QD to the acceptor ZnP have been systematically investigated, and an energy transfer efficiency over 70% in solution and a high optical sensitivity have been realized with the optimal of QD/ZnP ratio on films. A photocurrent density of up to 1.00×10-4 A·cm-2 can be achieved for the QD/ZnP film, and the corresponding on/off photocurrent value can be increased by two orders of magnitude compared with that of ZnP. Furthermore, the as-prepared light-harvesting film also demonstrates a high optical sensitivity at the optimal QD/ZnP ratio. Our research provides an effective way for designing and improving the QD/ZnP “antenna” for solar-energy harvesting devices.
关键词： Zinc Porphyrin,CsPbBr3 Quantum Dots,FRET,Photoresponse,Light-Harvesting Efficiency
更新于2025-09-19 17:13:59
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An enzyme-free FRET nanoprobe for ultrasensitive ketamine detection based on ATP-fueled target recycling
摘要： Ketamine is a commonly abused drug due to its stimulant, dissociative and hallucinogenic effects. An overdose of ketamine has been found to cause a variety of side effects. Therefore, the identification and quantification of ketamine are of significant importance for clinical purposes and drug seizing. However, conventional methods for ketamine detection possess some disadvantages such as sophisticated procedures, expensive instruments and low sensitivity. Herein, we develop a novel fluorescent nanoprobe for ultrasensitive ketamine detection with signal amplification based on Adenosine Triphosphate (ATP)-fueled target recycling and FRET (fluorescence resonance energy transfer) occurring between the FAM (Fluorescein, tagged with Y-shape DNA) and AuNPs. Based on the combination of FRET and signals circle amplification, the gold nanospheres functionalized with Y-motif DNA (Y@AuNPs) nanoprobe was utilized for effective ketamine detection with the limit of detection (LOD) down to 3 pg mL?1, which was lower than previously reported. Furthermore, the high sensitivity of Y@AuNPs facilitated quantitative analysis in biological media and practical samples.
关键词： Ultrasensitive detection,Ketamine,FRET,ATP-fueled target recycling,Fluorescent nanoprobe
更新于2025-09-16 10:30:52