研究目的
To explain the theory of QD-based FRET, review aspects of QD surface functionalization important for FRET, and discuss the advantages and disadvantages of QDs in FRET-biosensing using both spectroscopy and imaging techniques.
研究成果
QD-based FRET has become a versatile and powerful tool for the analysis of many different biomolecules and biomolecular interactions. The unique photophysical properties of QDs have significantly advanced optical biosensing beyond the possibilities of conventional fluorophores. QDs will continue to bring up completely new scientific ideas and applications in multiplexed and sensitive FRET biosensing, and will also be implemented as standard nanoparticle fluorophores to improve and advance existing research fields and technologies.
研究不足
The large size of QDs compared to molecular fluorophores can influence the conjugated biomolecule or biomolecules, altering binding functions and kinetics. The exact number of acceptors on the QD surface may be difficult to control, leading to a distribution of the overall FRET efficiency. QDs are prone to blinking, which influences the overall quantum yield and FRET efficiency in an ensemble experiment. The photophysical properties of QDs can be significantly influenced by the environment.
