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- 2017
- spectral reconstruction
- interference
- polarization
- transform
- imaging spectrometer
- Optoelectronic Information Science and Engineering
- BITTT
- Zhejiang University
- Guilin University of Aerospace Technology
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In vivo multiphoton microscopy detects longitudinal metabolic changes associated with delayed skin wound healing
摘要： Chronic wounds are difficult to diagnose and characterize due to a lack of quantitative biomarkers. Label-free multiphoton microscopy has emerged as a useful imaging modality capable of quantifying changes in cellular metabolism using an optical redox ratio of FAD/(NADH+FAD) autofluorescence. However, the utility of an optical redox ratio for long-term in vivo monitoring of tissue metabolism has not been robustly evaluated. In this study, we demonstrate how multiphoton microscopy can be used to monitor changes in the metabolism of individual full-thickness skin wounds in vivo. 3D optical redox ratio maps and NADH fluorescence lifetime images identify differences between diabetic and control mice during the re-epithelialization of wounds. These metabolic changes are associated with a transient increase in keratinocyte proliferation at the wound edge. Our study demonstrates that high-resolution, non-invasive autofluorescence imaging can be performed in vivo and that optical redox ratios can serve as quantitative optical biomarkers of impaired wound healing.
关键词： metabolism,optical redox ratio,autofluorescence,multiphoton microscopy,in vivo imaging,diabetes,FAD,NADH,wound healing
更新于2025-09-23 15:23:52
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BODIPY derivatives bearing borneol moieties: Enhancing cell membrane permeability for living cell imaging
摘要： Three novel boron-dipyrromethene (BODIPY) dyes bearing borneol moieties have been designed, synthesized and characterized. The single-crystal structure of the compound 3 was also elucidated for the first time. Their photophysical properties and confocal fluorescence images were investigated by the optical spectroscopy and Confocal fluorescence microscopy. The results indicate that the compounds 2-3 take on high fluorescence quantum yield and cell membrane permeability, which can be utilized as fluorescent visualizers for cell and lysosome fluorescence imaging.
关键词： photophysical properties,borneol,cell imaging,BODIPY,membrane permeability
更新于2025-09-23 15:23:52
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Synthesis, characterization and photophysical properties of meso-indole-boron-dipyrromethene derivatives and their cell imaging and viscosity sensing
摘要： Based on the molecular design and structural modification of boron-dipyrromethene (BODIPY), two new BODIPY derivatives with meso-substituted functional indole moieties were successfully prepared. These new compounds were characterized by NMR, HRMS and FTIR, exhibiting the different UV-vis and PL spectral phenomena because of their varied molecular structures in various solvents and different degrees of negative solvatochromism. Theoretical calculations and CV results show that the substituted NO2 group at 5-position of indole is favorable for increasing the oxidation potential of the molecules, while the NH2 group is the opposite. These two BODIPY derivatives have different sensitivities to pH changes, and can be appropriately applied as cell imaging materials due to their good physical chemical characteristics and excellent biocompatibility. In particular, the fluorescence characteristics of BODIPY derivative with the substituted NH2 group at 5-position of indole show a good solvent viscosity dependence, highlighting its potential application for testing the intracellular viscosity changes of the living cell in disease diagnosis.
关键词： meso-indole boron-dipyrromethene derivatives,photoluminescence,fluorescence characteristics,cell imaging,viscosity sensing
更新于2025-09-23 15:23:52
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Photoswitching FRET to monitor protein–protein interactions
摘要： FRET is a powerful approach to study the interactions of fluorescent molecules, and numerous methods have been developed to measure FRET in cells. Here, we present a method based on a donor molecule's photoswitching properties, which are slower in the presence vs. the absence of an acceptor. The technique, photoswitching FRET (psFRET), is similar to an established but underutilized method called photobleaching FRET (pbFRET), with the major difference being that the molecules are switched 'off' rather than photobleached. The psFRET technique has some of the FRET imaging advantages normally attributed to fluorescence lifetime imaging microscopy (FLIM), such as monitoring only donor fluorescence. However, it can be performed on a conventional widefield microscope, requires less illumination light to photoswitch off than photobleaching, and can be photoswitched 'on' again to repeat the experiment. We present data testing the validity of the psFRET approach to quantify FRET in cells and demonstrate its use in imaging protein–protein interactions and fluorescent protein-based biosensors.
关键词： FRET,imaging,microscopy,photoswitchable,fluorescence
更新于2025-09-23 15:23:52
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Polysiloxane-based Hyperbranched Fluorescent Materials Prepared by Thiol-ene “Click” Chemistry as Potential Cellular Imaging Polymers
摘要： Hyperbranched polymers have attracted increasing interest due to their unique properties which possess excellent RI and thermal stability, and have been widely used in fields of drug delivery, catalysts, liquid crystal and so on. Herein, polysiloxane-based hyperbranched fluorescent materials (P1 and P2) were synthesized by thiol-ene “click” chemistry. Then, novel polysiloxane-based hyperbranched fluorescent materials (P1-Ln3+) has been prepared by rare earth ions coordination. In view of the splendid fluorescence characteristics and favorable stability of P1-Ln3+, it has been applied in biological imaging. P1-Ln3+ has gained commendable applications in bioimaging.
关键词： Fluorescence materials,Living cells imaging,Hyperbranched polymers,Thiol-ene "click" chemistry,Coordination,Rare earth ions
更新于2025-09-23 15:23:52
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Blue-light imaging has an additional value to white-light endoscopy in visualization of early Barrett's neoplasia: an international multicenter cohort study
摘要： Endoscopic features of early neoplasia in Barrett’s esophagus (BE) are subtle. Blue-light imaging (BLI) may improve visualization of neoplastic lesions. The aim of this study was to evaluate BLI in visualization of Barrett’s neoplasia. Methods: Corresponding white-light endoscopy (WLE) and BLI images of 40 BE lesions were obtained prospectively and assessed by 6 international experts in 3 assessments. Each assessment consisted of overview and magnification images. Assessments were as follows: assessment 1, WLE only; assessment 2, BLI only; and assessment 3, corresponding WLE and BLI images. Outcome parameters were as follows: (1) appreciation of macroscopic appearance and surface relief (visual analog scale scores); (2) ability to delineate lesions (visual analog scale scores); (3) preferred technique for delineation (ordinal scores); and (4) quantitative agreement on delineations (AND/OR scores). Results: Experts appreciated BLI significantly better than WLE for visualization of macroscopic appearance (median 8.0 vs 7.0, P < .001) and surface relief (8.0 vs 6.0, P < .001). For both overview and magnification images, experts appreciated BLI significantly better than WLE for ability to delineate lesions (8.0 vs 6.0, P < .001 and 8.0 vs 5.0, P < .001). There was no overall significant difference in AND/OR scores of WLE + BLI when compared with WLE, yet agreement increased significantly with WLE + BLI for cases with a low baseline AND/OR score on WLE, both in overview (mean difference, 0.15; P = .015) and magnification (mean difference, 0.10; P = .01). Conclusions: BLI has additional value for visualization of BE neoplasia. Experts appreciated BLI better than WLE for visualization and delineation of BE neoplasia. Quantitative agreement increased significantly when BLI was offered next to WLE for lesions that were hard to delineate with WLE alone.
关键词： neoplasia,white-light endoscopy,visualization,Barrett's esophagus,delineation,blue-light imaging
更新于2025-09-23 15:23:52
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Monodimensional enlargement of resolved field of view in line scan cameras by a single doubly symmetric mirror
摘要： The geometry of the sensed and thus optically relevant area in the image plane of line scan cameras suggests that improvement of the overall system is possible, by joint design of optics and electronics. By a technique named Linear Fovea (LF) proposed in this paper, the optical contrast along the Line-of-Interest (LOI) in the image plane i.e. the location of the opto-electronic sensor, is optimized at the expense of contrast elsewhere. In the LF method, employing a single doubly symmetric surface, the resolved ?eld of view (RFOV) as the merit function, is maximized, by ?nding appropriate Zernike coe?cients describing that surface. The technique is applied on a proof-of-concept single mirror telescope and both MTF analysis and standard target imaging simulation are performed. Results show that by this technique, the RFOV can be doubled, or alternatively the illumination can be increased 2.5 times keeping RFOV unchanged. Furthermore, a rather uniform response is kept along the LOI.
关键词： Foveated imaging,Aberration compensation,Lens design,Wide ?eld angle lenses,Free form optics,Line scan cameras
更新于2025-09-23 15:23:52
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Local spatial correlation-based stripe non-uniformity correction algorithm for single infrared images
摘要： Stripe non-uniformity typically exists in infrared images and affects the visual effect; thus, eliminating stripe non-uniformity is essential to improve image quality. In this paper, a correction model with higher accuracy is developed. Unlike some other stripe non-uniformity correction methods using the same gain coefficient and offset parameters for the pixels of each column, different deviations of the correction parameters in the same column resulted by unsatisfactory preliminary non-uniformity correction are considered and are thought to be small and still relevant in space. The proposed method calculates the correction parameters for each pixel respectively based on the intrinsic spatial correlation between adjacent pixels in a column. What is more, an edge detection method is included. The experimental results indicate that the proposed algorithm effectively eliminates stripe noise of images of different scenes and it also works well in terms of preserving details. Furthermore, the algorithm exhibits high real-time performance.
关键词： De-striping,Spatial correlation,Non-uniformity correction,Stripe noise,Infrared imaging
更新于2025-09-23 15:23:52
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Fluorescent sensor for copper(II) ion based on coumarin derivative and its application in cell imaging
摘要： A novel fluorescent sensor CHT was designed based on the excellent optical properties of coumarin derivatives. It was synthesized by Schiff base reaction connected by 7-(N,N-diethylamino) coumarin-3-aldehyde and 2-hydrazinobenzothiazole. The sensor CHT displayed exhibited a significant fluorescence quenching at 540 nm upon the addition of Cu2+ ions. And the sensor exhibited good sensitivity, fast response time, high selectivity for Cu2+ ions in the presence of other important relevant metal ions. In addition, CHT fluorescent sensor was successfully applied for fluorescent imaging of Cu2+ ions in A549 and MCF-7 cells, demonstrating its potential applications in live cell imaging.
关键词： fluorescent sensor,coumarin,cell imaging,copper(II) ion
更新于2025-09-23 15:23:52
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Modern description of Rayleigh's criterion
摘要： Rayleigh’s criterion states that it becomes essentially difficult to resolve two incoherent optical point sources separated by a distance below the width of point spread functions (PSFs), namely, in the subdiffraction limit. Recently, researchers have achieved superresolution for two incoherent point sources with equal strengths, surpassing Rayleigh’s criterion. However, situations where more than two point sources needed to be resolved have not been fully investigated. Here we prove that for any incoherent sources with arbitrary strengths, a one- or two-dimensional (1D or 2D) image can be precisely resolved up to its second moment in the subdiffraction limit, i.e., the Fisher information (FI) is nonzero. But the FI with respect to higher order moments always tends to zero polynomially as the size of the image decreases, for any type of nonadaptive measurement. We call this phenomenon a modern description of Rayleigh’s criterion. For PSFs under certain constraints, the optimal measurement basis estimating all moments in the subdiffraction limit for 1D weak-source imaging is constructed. Such a basis also generates the optimal-scaling FI with respect to the size of the image for 2D or strong-source imaging, which achieves an overall quadratic improvement compared to direct imaging.
关键词： Fisher information,optical imaging,Rayleigh's criterion,superresolution,incoherent sources,subdiffraction limit
更新于2025-09-23 15:23:52