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A Single Simulation Platform for Hybrid Photoacoustic and RF-Acoustic Computed Tomography
摘要： In recent years, multimodal thermoacoustic imaging has demonstrated superior imaging quality compared to other emerging modalities. It provides functional and molecular information, arising due to electromagnetic absorption contrast, at ultrasonic resolution using inexpensive and non-ionizing imaging methods. The development of optical- as well as radio frequency (RF)-induced thermoacoustic imaging systems would benefit from reliable numerical simulations. To date, most numerical models use a combination of different software in order to model the hybrid thermoacoustic phenomenon. Here, we demonstrate the use of a single open source finite element software platform (ONELAB) for photo- and RF-acoustic computed tomography. The solutions of the optical diffusion equation, frequency domain Maxwell’s equations, and time-domain wave equation are used to solve the optical, electromagnetic, and acoustic propagation problems, respectively, in ONELAB. The results on a test homogeneous phantom and an approximate breast phantom confirm that ONELAB is a very effective software for both photo- and RF-acoustic simulations, and invaluable for developing new reconstruction algorithms and hardware systems.
关键词： tomography,thermoacoustic,radio frequency,photoacoustic imaging
更新于2025-09-23 15:22:29
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Aggregation-Induced Absorption Enhancement for Deep Near-Infrared II Photoacoustic Imaging of Brain Gliomas In Vivo
摘要： The delineation of brain gliomas margins still poses challenges to precise imaging and targeted therapy, mainly due to strong light attenuation of the skull and high background interference. With deep penetration and high sensitivity, photoacoustic (PA) imaging (PAI) in the second near-infrared (NIR II) window holds great potential for brain gliomas imaging. Herein, mesoionic dye A1094 encapsulated in Arg-Gly-Asp-modified hepatitis B virus core protein (RGD-HBc) is designed and synthesized for effective NIR II PAI of brain gliomas. An aggregation-induced absorption enhancement mechanism is discovered in vitro and in vivo. It is also demonstrated that A1094@RGD-HBc, with an enhanced absorption in the NIR II window, displays ninefold PA signal amplification in vivo, allowing for precise PAI of the brain gliomas at a depth up to 5.9 mm. In addition, with the application of abovementioned agent, high-resolution PAI and ultrasensitive single photon emission computed tomography images of brain gliomas are acquired with accurate co-localization. Collectively, the results suggest great promise of A1094@RGD-HBc for diagnostic imaging and precise delineation of brain gliomas in clinical applications.
关键词： mesoionic dyes,brain gliomas,aggregation-induced absorption enhancement,second near-infrared window,deep photoacoustic imaging
更新于2025-09-23 15:22:29
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Enhanced photoacoustic and photothermal effect of functionalized polypyrrole nanoparticles for near-infrared theranostic treatment of tumor
摘要： Functionalized nanomaterials with near-infrared (NIR) responsive capacity are quite promising for theranostic treatment of tumors, but formation of NIR responsive nanomaterials with enhanced theranostic ability and excellent biocompatibility is still very challenging. Herein, PEGylated indocyanine green (ICG)-loaded polypyrrole nanoparticles (PPI NPs) were designed and successfully formed through selecting polydopamine as the linkage between each component, demonstrating enhanced NIR responsive theranostic ability against tumor. Combining in vitro cell study with in vivo assay, the formed PPI NPs were proved being fantastic biocompatible while effectively internalizing in HeLa cells and retaining in HeLa tumor demonstrated by in vitro flow cytometry/confocal measurement and in vivo photoacoustic imaging assay. With the guidance of photoacoustic imaging, successful photothermal ablation of tumor was achieved when treating with PPI NPs plus laser, which was much more effective than the group treated with NPs free of ICG. The greatly combined enhanced photoacoustic and photothermal effect is mainly ascribed to the functionalized polypyrrole nanoparticles, which could accumulate in tumor site more effectively with a relative longer retention time taking advantage of the nanomaterial-induced endothelial leakiness phenomenon. All these results demonstrate the designed PPI NPs possess enhanced NIR responsive property are to hold a great promise for tumor NIR theranostic applications.
关键词： polypyrrole nanoparticles,photoacoustic imaging,photothermal therapy,Functionalized nanomaterials,near-infrared (NIR) responsive,theranostic treatment
更新于2025-09-23 15:21:21
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Switchable Photoacoustic Imaging of Glutathione Using MnO2 Nanotubes for Cancer Diagnosis
摘要： Glutathione is overexpressed in tumor cells and regulates cancer growth, metastasis and drug resistance. Therefore, detecting glutathione levels may greatly facilitate cancer diagnosis and treatment response monitoring. Photoacoustic (PA) imaging is a noninvasive modality for high-sensitivity, high-resolution, deep-tissue optical imaging. Switchable PA probes that offer signal on/off responses to tumor targets would further improve the detection sensitivity and signal-to-noise ratio of PA imaging. Here we explore the use of MnO2 nanotubes as a switchable and biodegradable PA probe for dynamic imaging of glutathione in cancer. Glutathione reduces black MnO2 nanotubes into colorless Mn2+ ions, leading to decreased and “signal off” PA amplitude. In phantoms, we observed a linear response of reduced PA signals of MnO2 nanotubes to increased glutathione concentrations. Using melanoma as the disease model, we demonstrated that MnO2 nanotubes-based PA imaging of glutathione successfully distinguished B16F10 melanoma cells from BEAS-2b normal cells and discriminated B16F10 tumors from healthy skin tissues. Our results showed that MnO2 nanotubes are a potent switchable and biodegradable PA probe for glutathione imaging in cancer diagnosis.
关键词： photoacoustic imaging,nanotubes,cancer,glutathione,melanoma,switchable contrast reagent,MnO2
更新于2025-09-23 15:21:21
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Novel Biodegradable Polymer Tethered Platinum (II) for Photoacoustic Imaging
摘要： Photoacoustic microscopy (PAM) is an emerging imaging diagnostic technique for various diseases. Coupled with contrast agents, photoacoustic (PA) imaging yields additional information to facilitate an accurate diagnosis. While many organic-based contrast agents, notably those of cyanine dyes, nanoparticles, polyhydroxy-fullerene and carbon nanotubes, have become available, the use of transition metal as contrast agent is scant. Here, for the first time, we report a platinum II-based biodegradable polymer for PA imaging that is capable of effective cellular internalization with very low cytotoxicity. The experiment results show great promise as a novel photoacoustic contrast agent as its detectable PA signal was observed both in cell imaging and in vivo rat cerebral vascular imaging via designed PAM. This work exemplifies the incorporation of transition metal complex with polymeric nanoparticles, further expanding the field of the ability of PA imaging.
关键词： Platinum,Biodegradable polymer,Photoacoustic imaging,Contrast agent,Transition metal
更新于2025-09-23 15:21:21
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Engineering Metal Organic Frameworks for Photoacoustic Imaging Guided Chemo/Photothermal Combinational Tumor Therapy
摘要： Imaging-guided therapy has considerable potential in tumor treatment. Different treatments have been integrated to realize combinational tumor therapy with improved therapeutic efficiency. Herein, the conventional metal organic framework (MOF) MIL-100 is utilized to load curcumin with excellent encapsulation capacity. Polydopamine-modified hyaluronic acid (HA-PDA) is coated on MIL-100 surface to construct engineering MOF nanoparticles (MCH NPs). The HA-PDA coating not only improves the dispersibility and stability of NPs but also introduces a tumor-targeting ability to this nanosystem. A two-stage augmented photothermal conversion capability is introduced to this nanosystem by encapsulating curcumin in MIL-100 pores and then coating HA-PDA on the surface, which confer the MCH NPs with strong photothermal conversional efficiency. After being intravenously injected into xenograft HeLa tumor-bearing mice, MCH NPs prefer to accumulate at the tumor site and achieve photoacoustic imaging-guided chemo/photothermal combinational tumor therapy, generating nearly complete tumor ablation. Engineering MOFs is an efficient platform for imaging-guided combinational tumor therapy, as confirmed by in vitro and in vivo evaluations.
关键词： curcumin,photoacoustic imaging,metal organic frameworks,chemotherapy,photothermal therapy
更新于2025-09-23 15:21:21
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Optimizing the light delivery of linear-array-based photoacoustic systems by double acoustic reflectors
摘要： Although linear transducer arrays have been intensely used in photoacoustic imaging, their geometrical shape constrains light illumination. Today, most linear array based photoacoustic systems utilize side-illumination geometry, which consists of two line fiber bundles attached to the side of the probe. The angled light illumination increases the light travel distance in deep tissue, consequently limiting the imaging depth. This issue was partially addressed by adding a right angle prism in front of the transducer. While this design makes the light illumination and acoustic detection co-axial, the transducer and the fiber bundles are orthogonal to each other, making the system inconvenient for handheld use. To overcome this limitation, here we propose a double-reflector design, in which the second reflector redirects the acoustic signals by another 90°, so that the transducer and the fiber bundle are now parallel to each other. In this design, both the transducer and fiber bundle output are fitted into a compact housing for convenient handheld imaging. To evaluate the efficiency of our design, we performed various phantom and human in vivo experiments. Our results demonstrate that the double-reflector design indeed provides deeper imaging depth and it also allows for easy imaging of objects with uneven surfaces.
关键词： handheld imaging,photoacoustic imaging,co-axial illumination,double-reflector design,linear transducer arrays
更新于2025-09-23 15:21:01
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Photoacoustic imaging for monitoring periodontal health: A first human study
摘要： The gold-standard periodontal probe is an aging tool that can detect periodontitis and monitor gingival health but is highly error-prone, does not fully characterize the periodontal pocket, and causes pain. Photoacoustic imaging is a noninvasive technique that can address these limitations. Here, a range of ultrasound frequencies between 16-40 MHz were used to image the periodontium and a contrast medium based on cuttlefish ink was used to label the pockets. A 40 MHz ultrasound frequency could spatially resolve the periodontal anatomy, including tooth, gum, gingival margin, and gingival thickness of tooth numbers 7-10 and 22-27. The photoacoustic-ultrasound measurements were more precise (0.01 mm) than those taken with physical probes by a dental hygienist. Furthermore, the full geometry of the pockets could be visualized with relative standard deviations of 10% (n = 5). This study shows the potential for non-invasive monitoring of periodontal health with photoacoustic-ultrasound imaging in the dental clinic.
关键词： periodontal disease,gingival thickness,periodontal charting,Photoacoustic imaging,melanin nanoparticles,diagnosis,pocket depth
更新于2025-09-23 15:21:01
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Quality improvement of acoustic-resolution photoacoustic imaging of skin vasculature based on practical synthetic-aperture focusing and bandpass filtering
摘要： We previously developed a compact acoustic-resolution photoacoustic (PA) imaging system with optical ?ber-based illumination, by which blood vessels in the rat skin were visualized in vivo. However, there were issues to be improved in the imaging characteristics: blurring in the out-of-focus region of the ultrasound sensor and degraded resolution due to low-frequency components in the PA signals. These are common limitations in acoustic-resolution PA imaging methods. In this study, we attempted to solve these problems by introducing signal processing methods based both on the synthetic-aperture focusing technique, which was applied only to signals in the detection zone of the sensor in the horizontal direction that was determined in advance in a phantom study, and on digital acoustic signal ?ltering with three speci?c frequency bands. Ex vivo and in vivo PA imaging of blood vessels in the rat skin and subcutaneous tissue showed the validity of the present signal processing methods.
关键词： photoacoustic imaging,bandpass ?ltering,skin vasculature,synthetic-aperture focusing
更新于2025-09-23 15:21:01
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Lanthanide-based Nanocomposites for Photothermal Therapy under Near Infrared Laser: Relationship between Light and Heat, Bio-stability and Reaction Temperature
摘要： In this research, typical organic/inorganic photothermal therapy (PTT) agents were designed with combination of up-conversion luminescent (UCL) or NIR II imaging rare earth nanomaterials for photoacoustic (PA)/UCL/NIR II imaging-guided PTT under NIR laser irradiation. The results show that: (1) PTT effect mainly comes from NIR absorption and partly from the UCL light conversion. (2) The visible UCL emission is mainly quenched by the NIR absorption of the coated PTT agent and partly quenched by the visible absorption, indicating the excitation may play a more important role than in the UCL emission process. (3) The bio-stability of the composite might be decided by the synthesized reaction temperature. Among the five inorganic/organic nanocomposite, UCNP@MnO2 is the most suitable candidate for cancer diagnosis and treatment due to its stimuli-response ability to the micro-acid environment of tumor cells and highest bio-stability. The composites generate heat for PTT after entering the tumour cells, and then the visible light emission gradually regains as MnO2 is reduced to colorless Mn2+ ions, thereby illuminating the cancer cells after the therapy.
关键词： Photothermal therapy,Upconversion luminescence imaging,Mito-tracker,Photoacoustic imaging,Inorganic/organic nanocomposite
更新于2025-09-23 15:19:57