研究目的
To develop engineering metal organic frameworks (MOFs) for photoacoustic imaging guided chemo/photothermal combinational tumor therapy.
研究成果
The engineered MCH NPs are promising candidates for imaging-guided chemo/photothermal combinational cancer therapy, as confirmed by in vitro and in vivo evaluations. The MCH NPs exhibited high curcumin-loading capability, improved stability, tumor-targeting ability, and strong photothermal conversion efficiency, leading to nearly complete tumor ablation in vivo.
研究不足
The study does not address the potential long-term toxicity of the MCH NPs or their biodegradability in vivo. The photothermal conversion efficiency, while comparable to other agents, could be further optimized.
1：Experimental Design and Method Selection:
The study utilized MIL-100 MOF to load curcumin and coated it with polydopamine-modified hyaluronic acid (HA-PDA) to construct MCH NPs. The methodology included microwave-assisted synthesis of MIL-100, loading of curcumin, and coating with HA-PDA.
2：Sample Selection and Data Sources:
HeLa cells, A549 cells, CHO cells, and MRC-5 cells were used for in vitro studies, and HeLa tumor-bearing mice were used for in vivo studies.
3：List of Experimental Equipment and Materials:
Equipment included a microwave reactor, UV-Vis spectrophotometer, infrared thermographic camera, and multispectral optoacoustic tomography (MSOT) system. Materials included FeCl3·6H2O, BTC, HA, curcumin, and dopamine hydrochloride.
4：Experimental Procedures and Operational Workflow:
The procedures included synthesis of MIL-100, curcumin loading, HA-PDA coating, photothermal effect measurement, drug release behavior study, stability tests, cellular uptake and cytotoxicity assays, in vivo imaging, and tumor therapy evaluation.
5：Data Analysis Methods:
Data were analyzed using statistical methods including Student’s t-test, with results presented as mean ± standard deviation.
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UV-Vis spectrophotometer
Shimadzu UV-2401PC
Shimadzu
Used for measuring the amount of curcumin in NPs.
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MIL-100
Metal organic framework used as a drug carrier with high drug-loading capacity.
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HA-PDA
Polydopamine-modified hyaluronic acid used for coating MIL-100 to improve dispersibility, stability, and introduce tumor-targeting ability.
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808 nm laser
MXL-III-880
Used for photothermal therapy and photoacoustic imaging.
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Infrared thermographic camera
Used to record temperature changes during photothermal therapy.
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Multispectral optoacoustic tomography (MSOT) system
Used for photoacoustic imaging in vivo.
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Guava easyCyte 6-2L Base System
Guava easyCyte 6-2L Base System
Merck Millipore
Used for flow cytometry to evaluate cellular uptake and apoptosis.
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Maestro In Vivo Imaging System
Maestro In Vivo Imaging System
Cambridge Research & Instrumentation, Inc.
Used for in vivo fluorescence imaging.
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ICP-MS
PerkinElmer
Used for determining the amount of Fe in blood samples.
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