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Effects of Carbon Arc Lamp Irradiation on Wound Healing in a Rat Cutaneous Full-Thickness Wound Model
摘要： Objective: The objective of the present study was to investigate the application of a carbon arc lamp on wound healing in a rat cutaneous full-thickness wound model. Background data: In clinical practice, wound healing has been promoted by irradiation with a carbon arc lamp. However, the corresponding mechanism has not been clearly defined. Methods: A cutaneous full-thickness wound on the back of rats was irradiated using a carbon arc lamp at a wavelength peak range of 620–740 nm with 54 J/cm2. Injured sham-irradiated control rats were used as the control. The rats were euthanized after 7, 14, and 21 days, while wound reepithelialization and healing quality were examined by histological analyses with comparison between groups. Cell proliferation was observed by 5-bromo-2￠-deoxyuridine (BrdU) immunohistochemical staining. Results: Irradiation by the carbon arc lamp significantly accelerated wound healing. The wound-healing rate in the treated group at day 21 was 98.42% – 0.56%, compared with 93.58% – 1.26% in the control group ( p < 0.05). Significant increases in the length of epithelial edges, collagen content, and microvessel density were observed in the wound sites in the treated group at days 7, 14, and 21 ( p < 0.05). Moreover, the number of BrdU-labeled cells increased in the wound edge at days 7 and 14 due to irradiation ( p < 0.05). Conclusions: The results demonstrated that the carbon arc lamp can promote wound healing together with improvement in its quality by stimulating cell proliferation.
关键词： wound healing,cell proliferation,carbon arc lamp
更新于2025-09-23 15:22:29
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Regulation of NADPH-dependent Nitric Oxide and reactive oxygen species signalling in endothelial and melanoma cells by a photoactive NADPH analogue
摘要： Nitric Oxide (NO) and Reactive oxygen species (ROS) are endogenous regulators of angiogenesis-related events as endothelial cell proliferation and survival, but NO/ROS defect or unbalance contribute to cancers. We recently designed a novel photoactive inhibitor of NO-Synthases (NOS) called NS1, which binds their NADPH site in vitro. Here, we show that NS1 inhibited NO formed in aortic rings. NS1-induced NO decrease led to an inhibition of angiogenesis in a model of VEGF-induced endothelial tubes formation. Beside this effect, NS1 reduced ROS levels in endothelial and melanoma A375 cells and in aorta. In metastatic melanoma cells, NS1 first induced a strong decrease of VEGF and blocked melanoma cell cycle at G2/M. NS1 decreased NOX4 and ROS levels that could lead to a specific proliferation arrest and cell death. In contrast, NS1 did not perturb melanocytes growth. Altogether, NS1 revealed a possible cross-talk between eNOS- and NOX4 – associated pathways in melanoma cells via VEGF, Erk and Akt modulation by NS1 that could be targeted to stop proliferation. NS1 thus constitutes a promising tool that modulates NO and redox stresses by targeting and directly inhibiting eNOS and, at least indirectly, NADPH oxidase(s), with great potential to control angiogenesis.
关键词： ROS,endothelium,NADPH analogue,Angiogenesis,melanoma,cell proliferation,Cellular signaling
更新于2025-09-19 17:15:36
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The Effect of Laser Structuring of Carbon Nanotubes on the Proliferation of Chondroblasts and Mesenchymal Stem Cells
摘要： The density of cartilage cells (chondroblasts) proliferating on a silicon substrate coated with vertically oriented arrays of multi-walled carbon nanotubes (MWCNTs) was shown to be higher than on a pure silicon substrate. Electron microscopy showed that the cells in a nutrient medium affected the vertical position of the nanotubes in the array. A method for structuring the MWCNT arrays by 100-ns laser pulse scanning and abrasive water processing on planar substrates was developed. As a result of the structuring of the MWCNTs, the arrays become resistant to bending under the influence of the nutrient medium with mesenchymal stem cells. Structured MWCNT arrays were shown to have no toxic or pathological effect on the viability and morphology of stem cells. Thus, such materials can be suggested for use in cell-adhesive components of biomedical devices.
关键词： carbon nanotubes,laser structuring,mesenchymal stem cells,chondroblasts,cell proliferation
更新于2025-09-19 17:13:59
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Sandwich-structure transferable free-form OLEDs for wearable and disposable skin wound photomedicine
摘要： Free-form optoelectronic devices can provide hyper-connectivity over space and time. However, most conformable optoelectronic devices can only be fabricated on flat polymeric materials using low-temperature processes, limiting their application and forms. This paper presents free-form optoelectronic devices that are not dependent on the shape or material. For medical applications, the transferable OLED (10 μm) is formed in a sandwich structure with an ultra-thin transferable barrier (4.8 μm). The results showed that the fabricated sandwich-structure transferable OLED (STOLED) exhibit the same high-efficiency performance on cylindrical-shaped materials and on materials such as textile and paper. Because the neutral axis is freely adjustable using the sandwich structure, the textile-based OLED achieved both folding reliability and washing reliability, as well as a long operating life (>150 h). When keratinocytes were irradiated with red STOLED light, cell proliferation and cell migration increased by 26 and 32%, respectively. In the skin equivalent model, the epidermis thickness was increased by 39%; additionally, in organ culture, not only was the skin area increased by 14%, but also, re-epithelialization was highly induced. Based on the results, the STOLED is expected to be applicable in various wearable and disposable photomedical devices.
关键词： Keratinocytes,Wearable photomedicine,Skin wound healing,Cell migration,Epidermis thickness,Re-epithelialization,Free-form optoelectronic devices,Sandwich-structure transferable OLED,Cell proliferation
更新于2025-09-12 10:27:22
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Effects of diamond-like carbon thin film and wrinkle microstructure on cell proliferation
摘要： This paper aims to clarify the effects of diamond-like carbon (DLC) thin film and the wrinkle microstructure formed by depositing the DLC thin film on a poly(dimethylsiloxane) (PDMS) substrate on cell proliferation with a view to applying to cell scaffolds, which are essential technologies to control cellular activity and behaviors for cell therapy and therapeutic applications. Mouse myoblast cell (C2C12) culture tests using substrates with/without the DLC thin film and the wrinkle microstructure revealed that the cell proliferation was largely enhanced by the existence of the DLC thin film on the substrate. Moreover, it was examined how the size of the wrinkle of the DLC thin film deposited PDMS substrates affected cellular growth, and it was found that the wrinkle microstructure had the optimum range of the height difference of the wrinkle, from 0.7 to 1.0 μm as the surface roughness (Rc), for promoting cell proliferation.
关键词： Wrinkle microstructure,PDMS,Diamond-like carbon,Cell proliferation
更新于2025-09-11 14:15:04
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Arabidopsis E2Fc is required for the DNA damage response under UV-B radiation epistatically over the microRNA396 and independently of E2Fe
摘要： UV-B radiation inhibits plant growth, and this inhibition is, to a certain extent, regulated by miR396-mediated repression of Growth Regulating transcription Factors (GRFs). Moreover, E2Fe transcription factor also modulates Arabidopsis leaf growth. Here, we provide evidence that, at UV-B intensities that induce DNA damage, E2Fc participates in the inhibition of cell proliferation. We demonstrate that E2Fc deficient plants show a lower inhibition of leaf size under UV-B conditions that damage DNA, decreased cell death after exposure and altered SOG1 and ATR expression. Interestingly, the previously reported participation of E2Fe in UV-B responses, which is a transcriptional target of E2Fc, is independent and different of that described for E2Fc. On the other hand, we here demonstrate that E2Fc has an epistatic role over the miR396 pathway under UV-B conditions. Finally, we show that inhibition of cell proliferation by UV-B is independent of the regulation of class II TCP transcription factors. Together, our results demonstrate that E2Fc is required for miR396 activity on cell proliferation under UV-B, and that its role is independent of E2Fe, probably modulating DNA damage responses through the regulation of SOG1 and ATR transcript levels.
关键词： E2F transcription factor,DNA damage response,UV-B,cell proliferation,miR396
更新于2025-09-10 09:29:36
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Antiviral Drug Ganciclovir Is a Potent Inhibitor of the Proliferation of Müller Glia–Derived Progenitors During Zebrafish Retinal Regeneration
摘要： PURPOSE. The purpose of this study was to investigate the effect of the antiviral drug ganciclovir (GCV) on M¨uller glia dedifferentiation and proliferation and the underlying cellular and molecular mechanisms in adult zebra?sh. METHODS. A Tg(1016tuba1a:GFP) transgenic line was generated to identify injury-induced dedifferentiation of M¨uller glia. Mechanical retinal damage was induced by a needle-poke injury on the back of the eyes in adult zebra?sh. Phosphate-buffered saline or GCV was injected into the vitreous of the eye at the time of injury or through the cornea. The GCV clearance rate from the eye was determined by a reversed-phase HPLC method. Green ?uorescent protein (GFP) and bromodeoxyuridine (BrdU) immuno?uorescence were used to determine the effect of GCV on retinal regeneration. Cell apoptosis was evaluated by TUNEL staining. Microglia were labeled by vitreous injection of isolectin IB4 conjugates. Quantitative (q)PCR and Western blot analysis were used to determine gene expression in the retina. RESULTS. Ganciclovir treatment signi?cantly reduced the number of BrdUt M¨uller glia–derived progenitor cells (MGPCs) at 4 days post injury. Further analysis showed that GCV had no impact on M¨uller glia dedifferentiation and the initial formation of MGPCs. Our data indicate that GCV irreversibly inhibited MGPC proliferation likely through a p53-p21cip1–dependent pathway. Interestingly, unlike control cells, GCV-treated M¨uller glia cells were ‘‘locked’’ in a prolonged dedifferentiated state. CONCLUSIONS. Our study uncovered a novel inhibitory effect of GCV on MGPC proliferation and suggests its potential use as a tool to uncover molecular mechanisms underlying retinal regeneration in zebra?sh.
关键词： cell proliferation,M¨uller glia,retinal regeneration,ganciclovir,zebra?sh
更新于2025-09-10 09:29:36
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Image Analysis in Digital Pathology: Combining Automated Assessment of Ki67 Staining Quality with Calculation of Ki67 Cell Proliferation Index
摘要： The human Ki-67 antigen first described by Gerdes et al. in 1983 is now well established as an indicator of cell proliferation and has been shown to be a strong prognostic marker for several cancer types. It is in particular commonly used for assessment of early breast cancer. Unfortunately no standard Ki67 staining protocol exists, resulting in inter-laboratory stain variability. This may lead to errors in the assessment when the Ki67 index is evaluated. Therefore, it is important to establish some form of quality control of a staining protocol to ensure correct diagnosis and treatment of patients. In this issue of Cytometry Laang and coworkers presents a procedure for determining staining quality through image analysis of cell nuclei from cell lines. The image analysis-based assessment is related to an expert panel-based subjective staining quality assessment system provided by an organization called NordiQC. The result from the analysis presented in the article is that staining quality can be estimated automatically by image analysis of cells from cell lines with known Ki67 index with good correlation to the human expert evaluation of staining quality. And it is concluded that “The estimated stain quality can then give the pathologist a better opportunity to interpret the tissue sample and may aid in reducing intra- and inter-observer variability in analysis and diagnosis of tissue samples with Ki67 staining.”
关键词： image analysis,cell proliferation,digital pathology,Ki67,staining quality
更新于2025-09-10 09:29:36
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[IEEE 2018 9th International Conference on Ultrawideband and Ultrashort Impulse Signals (UWBUSIS) - Odessa, Ukraine (2018.9.4-2018.9.7)] 2018 9th International Conference on Ultrawideband and Ultrashort Impulse Signals (UWBUSIS) - The Application of Pulsed Electric Fields and Other Types of Electromagnetic Radiation in Therapy of Cancer
摘要： since the beginning of 21-th century the investigations in the area of cancer therapy by electric fields become more numerous. These investigations may be divided in three groups: studies carried out on cell cultures, studies on animal models and case reports describing applications of pulsed electric fields for treatment of cancer patients. Very important is to chose adequate methods of measuring the cell damage and speaking more generally – cell response to stress. In Kharkov University various investigations in the area of cell response to electromagnetic influences were carried out and some new methods were proposed. In this paper the analysis of recent works in this area is done and perspectives of the research are discussed.
关键词： electromagnetic pulse,cell proliferation,cell membrane permeability,cell response to electromagnetic stress,apoptosis,cancer therapy,necrosis
更新于2025-09-09 09:28:46