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A molecularly imprinted modified CdSeS/ZnS core–shell quantum dot embedded glass slide for highly selective and sensitive solid phase optosensing of trace amounts of lidocaine in biological samples
摘要： An optosensing material based on surface functionalization of a glass slide by quantum dots (QDs) and molecularly imprinted polymer (glass slide@QDs@MIP) with unique optical properties of QDs and selective recognition of MIPs was fabricated for the determination of lidocaine in biological samples. Firstly, CdSeS/ZnS quantum dots were covalently attached to a glass slide surface. Then a molecularly imprinted polymer layer, using lidocaine as the template, was embedded onto the modified surface of the glass slide@QDs by copolymerization to prepare a solid phase glass slide@QDs@MIP sensor. The proposed sensor was more strongly quenched by lidocaine than the non-imprinted polymer (glass slide@QDs@NIP) indicating selective recognition of lidocaine by glass slide@QDs@MIP. The main effecting parameters on the extraction and recognition of lidocaine such as the pH of the sample solution and reaction time were investigated and optimized. Under optimal conditions, the synthesized MIP optosensing sensor offered good response for lidocaine with the linear range varying from 0.002-1.0 μM and a correlation coefficient of 0.9991. The limit of detection (3Sb/m) of the method and limit of quantification (10Sb/m) were 6.3 × 10-4 and 2.0 × 10-3 μM, respectively. The intra-day and inter-day RSDs were 1.9% and 4.4%, respectively. The proposed sensor was practically applicable to detection a trace amount of lidocaine in real biological samples (urine and plasma) with satisfactory recoveries in the range of 96.7-105.4% and RSDs between 1.8-4.9%. The sensor showed obvious advantages such as photostability, simple and rapid detection of lidocaine in complex matrices and specific recognition toward lidocaine.
关键词： lidocaine,biological samples,molecularly imprinted polymer,optosensing,quantum dots
更新于2025-11-19 16:46:39
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Molecularly Imprinted Polymer Integrated Plasmonic Nanosensor for Cocaine Detection
摘要： A molecularly imprinted polymeric nanofilm was prepared for cocaine detection and applied to plasmonic nanosensor for real-time kinetic, selectivity and reusability analyses. The sensing polymeric surface was fabricated by synthesizing a selective and specific nanofilm on the gold plasmonic nanosensor surface. After characterization experiments with atomic force microscopy, ellipsometer, and contact angle measurements, the kinetic studies of cocaine detection in aqueous solutions in a wide concentration range between 0.2-100 μg/mL were applied to plasmonic nanosensor system at 24oC with a low limit of detection (0.1 μg/L) and quantification values (0.3 μg/L) and the results showed that this molecularly imprinted polymeric nanofilm integrated plasmonic nanosensor is providing a model for the fastest, most accurate and most precise identification of the cocaine molecule which constitutes a large part of the workload of forensic laboratories.
关键词： nanofilm,Cocaine detection,molecularly imprinted polymer,plasmonic nanosensor
更新于2025-09-23 15:21:01
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Organic-inorganic nanoparticles molecularly imprinted photoelectrochemical sensor for α-Solanine based on p-Type Polymer Dots and n-CdS heterojunction
摘要： In this study, a molecularly imprinted polymer photoelectrochemcal (MIP-PEC) sensor based on semiconducting organic polymer dots (Pdots) and inorganic CdS quantum dots (QDs) has been established for the determination of α-Solanine. Specifically, p-type Pdots (p-Pdots) and n-type CdS QDs (n-CdS) were utilized to form organic-inorganic nanoparticles p-n heterojunction to enhance signal response, and their specific energy levels (VB/CB or HOMO/LUMO) were calculated for photoelectrochemical (PEC) bioanalysis application. At the same time, the combination of molecular imprinting technology and photoelectrochemistry overcomes the defeats of photoelectrochemistry which is the absence of selectivity, offers a new MIP-PEC sensor with high sensitivity and excellent selectivity based heterojunction enhanced strategy. In short, this study proposes the semiconducting organic-inorganic nanoparticles p-n heterojunction for molecularly imprinted polymer photoelectrochemcal bioanalysis application, and the MIP-PEC sensor was successfully fabricated based on these materials and methods. In the phosphate buffer solution (PBS), it was clearly observed that the photocurrent has a significant change between elution in acetic acid-ethanol mixture and incubation in template molecular solution because of the faster electron transfer speed, this phenomenon fully showed that the MIP-PEC sensor can specifically detect the target. Thus, the work typically offers a linear range from 0.01 to 1000 ng·mL?1 with a detection limit of 6.5 pg mL?1 for α-Solanine. Furthermore, the fabricated MIP-PEC sensor will confirm the actual application.
关键词： photoelectrochemical sensor,Molecularly imprinted polymer,p-n heterojunction,α-Solanine,organic-inorganic
更新于2025-09-19 17:15:36
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Synthesis of Multi-mode Quantum Dots Encoded Molecularly Imprinted Polymers Microspheres and Application in Quantitative Detection for Dopamine
摘要： As the promising analytical strategy, multi-mode quantum dots (QDs) encoded molecularly imprinted fluorescent sensors have attracted considerable attention. Here, a novel encoding strategy for water-dispersed QDs molecularly imprinted polymers microspheres (MIPMs) with a high-performance fluorescence intensity and multi-color signal was established. The water-compatible MIPMs were firstly synthesized via integrating the reversible addition-fragmentation chain transfer and precipitation polymerization (RAFT-PP), which could improve the water compatibility by grafting the hydrophilic groups of poly(2-hydroxyrthyl methacrylate) (PHEMA). Then the water-compatible MIPMs were used as embedding matrix to separately incorporate five different QDs, and a series of multi-color QDs encoded MIPs (five single-color and 10 dual-color fluorescent systems) were obtained. The fluorescent characterization of encoded MIPMs was studied in detail. Further, dual-mode fluorescent sensors, including monochromatic and ratiometric, were successfully fabricated for the quantitative detection of dopamine; good linear response in the range of 5-300 μg L-1 and 1-100 μg L-1 and the limit of detection (LOD) with 2 μg L-1 and 0.5 μg L-1 were obtained for monochromatic and ratiometric sensor, respectively. It is the first time to introduce the optical encoding strategy into the fabrication of fluorescent MIPs, which not only greatly simplifies the preparation process of fluorescent imprinted sensor, but also provides more alternatives for the detection method of analyte and the potential of high-throughput multiple analytes at one time.
关键词： Molecularly imprinted polymer,Quantum dot,Fluorescent sensor,Dopamine,Encoded microspheres
更新于2025-09-19 17:13:59
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Laser-Induced Graphene-Based Platforms for Dual Biorecognition of Molecules
摘要： Expanding the single molecule detection, enabled by laser-induced graphene (LIG) technology, for portable and on-site analysis, a dual molecule system with a two-working electrode architecture was developed, for ascorbic acid (AA) and amoxicillin (AMOX) detection, which are currently used in aquaculture and persist as water contaminants. The biorecognition element of each target compound was a suitable molecularly-imprinted polymer (MIP). The AMOX MIP was developed herein for the first time and assembled by electropolymerization of eriochrome black T (EBT). It showed a wide linear response from 100 nM to 50 μM, with a sensitivity of -13.32 μA/decade. Calibration curves revealed good squared correlation coefficients (R2 > 0.99) with a limit of detection (LOD) of 11.98 nM. AA MIP was assembled according to previous work reported in the literature, displaying a linear response from 1.5 mM to 4 mM and a sensitivity of 1.356 μA/decade. The developed dual-LIG device was further tested in real samples and successfully applied to the analysis of binary mixtures prepared in environmental water samples from a well. Overall, the proposed device allows in-situ analysis of two different molecules, holds an exceptionally low-cost design when compared to competing architectures in the literature, and, the fabrication method here employed offers the possibility of easily adjusting the desired architecture on demand.
关键词： Amoxicillin,Ascorbic acid,Laser-induced graphene,Molecularly-imprinted polymer technology,Dual detection
更新于2025-09-19 17:13:59
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Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers
摘要： Herein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularly-imprinted technology (MIP), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation. For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N′-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTe@MPA QDs is quenched in a concentration dependent manner. Using surface imprinting technology, the optimal fluorescence signals yielded a linear response versus logarithm of IL-2 concentration from 35 fg/ml to 39 pg/ml, in a 1000-fold diluted synthetic human serum. The limit of detection obtained is 5.91 fg/ml, lying below the concentration levels of IL-2 with clinical interest for cancer diagnosis (9.4-19.2 pg/ml). Overall, the method presented herein is a demonstration that the combination of MIP and QDs for protein detection constitutes a powerful tool in clinical analysis, providing low cost, sensitive and quick responses. The same concept may be further extended to other proteins of interest.
关键词： interleukin-2,conjugated-QDs,protein,molecularly imprinted polymer,Quantum dots,cancer biomarker
更新于2025-09-16 10:30:52
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An eco-friendly imprinted polymer based on graphene quantum dots for fluorescent detection of <i>p</i> -nitroaniline
摘要： An eco-friendly fluorescent molecularly imprinted polymer anchored on the surface of graphene quantum dots (GQDs@MIP) was developed with an efficient sol–gel polymerization for highly sensitive and selective determination of p-nitroaniline (p-NA). The GQDs@MIP was characterized in detail by Fourier-transform infrared, fluorescence spectrometer, scanning electron microscope, transmission electron microscope and ultraviolet spectrophotometer. The results showed that the imprinted layer was successfully grafted on the surface of the GQDs. The fluorescence of the GQDs@MIP is efficiently quenched when p-NA recombines with the imprinting sites based on the photo-induced electron transfer fluorescence quenching mechanism. A good linear relationship was obtained between the fluorescence quenching efficiency of the GQDs@MIP and the concentration of p-NA in the range of 0–15.0 mM with a correlation coefficient of 0.99. The practicability of the proposed method in real samples was successfully evaluated through monitoring p-NA in water and fish samples with satisfactory recovery. The developed method provides a feasible and eco-friendly strategy to fabricate MIPs anchored on GQDs with good fluorescence properties for sensitive detection of organic pollutants in complex samples.
关键词： graphene quantum dots,fluorescent molecularly imprinted polymer,p-nitroaniline,fluorescence quenching,eco-friendly
更新于2025-09-16 10:30:52
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Simple and sensitive molecularly imprinted polymer-MPA capped Mn-doped ZnS quantum dots-based phosphorescence probe for cyanotoxins determination in algae metabolites
摘要： This paper aimed to develop a simple method for detection and quantification of cylindrospermopsin (CYN) in the water sample. The novel biosensor was based on molecular imprinted Mn-doped ZnS quantum dots. Two fragments which mimicked the partial structure of CYN were carefully selected to synthesize the molecularly imprinted 3-mercaptopropionic acid-capped Mn-ZnS quantum dots (MPA-MnZnS-QDs-MIPs). As the two fragment imprinted sites could enhance the rebinding of CYN into them and modulated Mn-ZnS QDs’ selective aggregation in MPA-MnZnS-QDs-MIPs. Hence, the enhanced room temperature phosphorescence (RTP) response was observed as the MPA-MnZnS-QDs-MIPs interacted with CYN in the water sample. A good linear relationship with the concentration of CYN in the range of 30–320 μg L?1 with a detection limit of 24 μg L?1 was demonstrated by the enhanced phosphorescence intensity ratio ΔP/P0. And our detection method exhibited higher selectivity for CYN than the two partial fragments. Also, there was no interference on the detection system when the competitors were present in the algae culture samples. The method presented required minimal expertise and enabled fast and inexpensive monitoring of the water toxins.
关键词： Molecularly imprinted polymer,Cyanotoxins,Sensor,Quantum dots
更新于2025-09-16 10:30:52
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Orthogonal dual molecularly imprinted polymer-based plasmonic immunosandwich assay: A double characteristic recognition strategy for specific detection of glycoproteins
摘要： Sensitive and specific detection methods are critical to the detection of glycoproteins. Immunoassay has been a powerful tool for this purpose, in which antibodies or their mimics particularly molecularly imprinted polymers (MIPs) are used for specific recognition. Epitope and glycan are two structure features of a glycoprotein. However, immunoassays based on simultaneous recognition towards the two characteristics have been scarcely explored so far. Herein we present a new strategy called orthogonal dual molecularly imprinted polymer-based plasmonic immunosandwich assay (odMIP-PISA). It relies on double recognition towards a target glycoprotein by two different types of MIPs, using epitope-imprinted gold nanoparticles (AuNPs)-coated slide as capturing substrate to recognize the peptide epitope and glycans-imprinted Raman-active silver nanoparticles as labeling nanotags to recognize the glycans. Carcinoembryonic antigen (CEA), a routinely used marker for colon cancer, was used as a test glycoprotein. The orthogonal double recognition apparently improved the specificity, reducing the maximum cross-reactivity from 14.4% for epitope recognition and 15.2% for glycan recognition to 8.2% for double recognition. Meanwhile, the plasmonic nanostructure-based Raman detection provided ultrahigh sensitivity, yielding a limit of detection of 5.56×10-14 M (S/N = 10). Through measuring the CEA level in human serum, this method permitted differentiation of colon cancer patient from healthy individual. Compared with the traditional immunoassay, odMIP-PISA exhibited multiple advantages, including simplified procedure (6 steps), speed (30 min), reduced cost, and so on. Therefore, this new approach holds great promise in many applications particularly clinical diagnosis.
关键词： Raman spectroscopy,glycoprotein,plasmonic immunosandwich assay,molecularly imprinted polymer,epitope,glycosylation
更新于2025-09-12 10:27:22
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Surface Molecularly Imprinted Carbon Dots Based Core-Shell Material for Selective Fluorescence Sensing of Ketoprofen
摘要： In this work, we report an environment friendly core-shell material based on Carbon Dot core and Molecularly Imprinted Polymer shell as sensor for highly selective fluorescence detection of ketoprofen. The Carbon Dots (CDs) were prepared by a hydrothermal method and the polymer layer around the CDs core was synthesised by sol-gel polymerisation. The prepared material was characterized by Fluorescence Spectroscopy, FT-IR Spectroscopy and Transmission Electron Spectroscopy (TEM). Fluorescence from the Carbon Dots- Molecularly Imprinted Polymer (CDs-MIP) was found to quench selectively in the presence of ketoprofen and quenching effect was found to be greater than for Non-Imprinted Polymer (CDs-NIP) which indicated the potential of CDs-MIP as a fluorescence sensing material for ketoprofen. The imprinting factor was obtained to be 2.35. Under optimized conditions, a linear response was obtained in the concentration range from 0.039 to 3.9 μM with a detection limit of 0.01 μM. The correlation coefficient was 0.999. The developed sensor was applied to determination of ketoprofen in human serum and urine samples with good recoveries ranging from 96 to 104% indicating successful application of the proposed sensor in biological fluids.
关键词： Molecularly imprinted polymer,Fluorescence determination,Carbon dots,Ketoprofen
更新于2025-09-10 09:29:36