- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Enhanced surface plasmon resonance (SPR) signals based on immobilization of core-shell nanoparticles incorporated boron nitride nanosheets: Development of molecularly imprinted SPR nanosensor for anticancer drug, etoposide
摘要： An effective SPR nanosensor based on core-shell nanoparticles (Ag@AuNPs) incorporated hexagonal boron nitride (HBN) nanosheets and molecularly imprinted polymer (MIP) was presented for etoposide (ETO) detection. Scanning electron microscope (SEM), transmission electron microscope (TEM), x-ray diffraction (XRD) method, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), fourier transform infrared (FTIR) spectroscopy and atomic force microscopy (AFM) methods were utilized for all characterizations of nanomaterials and polymer surfaces. ETO imprinted SPR nanosensor based on Ag@AuNPs-HBN nanocomposite was developed in the presence of poly(2-hydroxyethyl methacrylate-methacryloylamidoglutamic acid) [p(HEMA-MAGA)]. The results of the study have revealed that 0.001 - 1.00 ng mL-1 (1.70×10-12 - 1.70×10-9 M) and 0.00025 ng mL-1 (4.25×10-13 M) were found as the linearity range and the detection limit (LOD). Furthermore, the prepared SPR nanosensor was examined in terms of stability, repeatability and selectivity. Finally, the imprinted SPR nanosensor was applied to the urine samples having high recovery.
关键词： Etoposide,Core-shell nanoparticles,Hexagonal boron nitride,Urine sample,Molecular imprinted nanosensor
更新于2025-11-21 11:18:25
-
Molecularly imprinted based surface plasmon resonance nanosensors for microalbumin detection
摘要： Human serum albumin (HSA) is a major blood plasma protein also found in urine where its existence may be a marker of some types of liver or kidney dysfunction. Herein, we fabricated a novel surface plasmon resonance (SPR) nanosensor for selective, sensitive, and label-free microalbumin detection both in aqueous and urine sample solutions. Firstly, HSA-imprinted nanoparticles were synthesized, which consist of ethylene glycol dimethacrylate and N-methacryloyl-L-leucine as a cross-linker and functional monomer. The nanoparticles were characterized by zeta-size and scanning electron microscope analyses and were dropped onto the SPR chip surface to make HSA sensitive nanosensor. Characterization studies of HSA-imprinted SPR chip were carried out by atomic force microscopy, Fourier-transform infrared spectroscopy, contact angle, and ellipsometer. The limit of detection and limit of quantification values of HSA-imprinted SPR nanosensor were calculated as 0.7 pM and 1.9 pM for the concentration range of 0.15-500 nM. Selectivity studies of HSA-imprinted SPR nanosensor were achieved with hemoglobin and transferrin proteins which were chosen as competitor molecules. HSA-imprinted SPR nanosensor was displayed highly selective and sensitive to HSA.
关键词： surface plasmon resonance,nanosensor,nanoparticles,microalbumin detection
更新于2025-11-21 11:03:13
-
Bifunctional gold nanoclusters enable ratiometric fluorescence nanosensing of hydrogen peroxide and glucose
摘要： The accurate quantification of hydrogen peroxide (H2O2) and glucose is essential significance in clinical diagnosis. Herein a selective and sensitive ratiometric fluorescent nanosensor was developed for the determination of H2O2 and glucose by integrating peroxidase–like catalytic and fluorescent bifunctional properties of glutathione protected gold nanoclusters (GSH–AuNCs). The GSH–AuNCs exhibit inherent peroxidase–like activity and accelerate the decomposition of H2O2 into hydroxyl radicals. The produced hydroxyl radicals oxidize terephthalic acid (TA), a typical non–fluorescent substrate of peroxidase, to a highly fluorescent product hydroxyterephthalate (TAOH). Upon excitation with single–wavelength at 315 nm, dual–emission fluorescence peaks were recorded at 430 and 600 nm simultaneously. The fluorescence signal of TAOH at 430 nm continuously increased with increasing the concentration of H2O2 while the fluorescence signal of GSH–AuNCs at 600 nm remained unchangeable. Based upon on these facts, a ratiometric fluorescent nanosensor was fabricated for H2O2 assay with TAOH as response unit and GSH–AuNCs as reference, respectively. By converting glucose into H2O2 with catalytic oxidation of glucose oxidase (GOx), this nanosensor was further exploited for glucose assay. Under the optimum conditions, the detection limits of 10 nmol/L H2O2 and 20 nmol/L glucose were acquired. The relative standard deviations were less than 5% for both H2O2 and glucose (5.0 μmol/L solution, n = 11). The practicability of the nanosensor was verified by the determination of glucose in human serum samples. This nanosensor can be easily expanded as a general platform for the detection of other substances involving H2O2 produced or consumed.
关键词： Intrinsic peroxidase-like activity,Fluorescent gold nanoclusters,Hydrogen peroxide,Glucose,Ratiometric fluorescence,Nanosensor
更新于2025-11-14 17:03:37
-
Amino-Functionalized Al-MOF for Fluorescent Detection of Tetracyclines in Milk
摘要： A fluorescent method for detection of tetracyclines (TCs) in milk was developed by using the NH2-MIL-53(Al) nanosensor synthesized via a one-pot hydrothermal method. The nanosensor had a crystalline nanoplates structure with rich groups of -NH2 and -COOH. The -NH2/-COOH of NH2-MIL-53(Al) reacted with the -CO-/-OH of TCs to form a complex. The electron of -NH2/-COOH from the NH2-BDC ligand transferred to the -CO-/-OH of TCs. -NH2 of the NH2-MIL-53(Al) interacted with the -CO-/-OH of TCs by hydrogen bonding. Quenching efficiency of inner filter effect (IFE) was calculated to contribute 57-89%. The synergistic effect of photoinduced electron transfer (PET) and IFE account for fluorescence quenching. TCs were quantitatively detected in milk samples with recoveries of 85.15 ~ 112.13%, the results were in great accordance with high performance liquid chromatography (HPLC) (P > 0.05), confirming the NH2-MIL-53(Al) nanosensor has potential applicability for the detection of TCs in food matrix.
关键词： Nanosensor,inner filter effect (IFE),Amino-functionalized organic Metal Framework,Tetracyclines,Fluorescence detection,Milk
更新于2025-09-23 15:23:52
-
Bi-functional heater-thermometer Nd3+-doped nanoparticles with multiple temperature sensing parameters
摘要： Achieving a combination of real-time diagnosis and therapy in a single platform with sensitive thermometry and efficient heat production is a crucial step towards controllable photo-thermal therapy. Here, Nd3+-doped Y2O3 nanoparticles prepared by combined Pechini-foaming technique operating in first and second biological windows were demonstrated as thermal sensors within wide temperature range of 123–873 K and heaters with temperature increase by 100 K. Thermal sensing was performed based on various approaches: luminescence intensity ratio (electronic levels; Stark sublevels), spectral line position and line bandwidth were used as temperature dependent parameters. Applicability of regarded sensing parameters along with relative thermal sensitivity and temperature resolution were discussed and compared. Influence of Nd3+ doping concentration on thermometer and heater efficiency was also investigated.
关键词： Nanosensor,Biological window,Hyperthermia,Luminescence,Thermometry,Nd3+
更新于2025-09-23 15:23:52
-
Sensors Based on Metal Nanoclusters Stabilized on Designed Proteins
摘要： Among all new nanomaterials, metal nanoclusters (NCs) have attracted special attention due to their interesting optical properties, among others. Metal NCs have been recently studied and used as sensors for different analytes. However, there is a need to explore the potential of these new sensors in a systematic manner and to develop new systems to broaden the possibilities that sensing offers to the industry. In this work, we show the potential use of repeat protein scaffolds as versatile templates for the synthesis and stabilization of various metal NCs, speci?cally Au, Ag, and CuNCs. The resulting protein-metal NCs hybrids are evaluated as sensors for different stimuli such as temperature, ions, or reactive oxygen species (ROS). Among the three protein-metal NCs, all performed nicely as temperature sensors, AuNCs responded to metal ions, and AgNCs were able to detect ROS.
关键词： nanosensor,ROS sensors,metal nanocluster,protein design,temperature sensor,metal sensors,?uorescent probe
更新于2025-09-23 15:21:21
-
Molecularly Imprinted Polymer Integrated Plasmonic Nanosensor for Cocaine Detection
摘要： A molecularly imprinted polymeric nanofilm was prepared for cocaine detection and applied to plasmonic nanosensor for real-time kinetic, selectivity and reusability analyses. The sensing polymeric surface was fabricated by synthesizing a selective and specific nanofilm on the gold plasmonic nanosensor surface. After characterization experiments with atomic force microscopy, ellipsometer, and contact angle measurements, the kinetic studies of cocaine detection in aqueous solutions in a wide concentration range between 0.2-100 μg/mL were applied to plasmonic nanosensor system at 24oC with a low limit of detection (0.1 μg/L) and quantification values (0.3 μg/L) and the results showed that this molecularly imprinted polymeric nanofilm integrated plasmonic nanosensor is providing a model for the fastest, most accurate and most precise identification of the cocaine molecule which constitutes a large part of the workload of forensic laboratories.
关键词： nanofilm,Cocaine detection,molecularly imprinted polymer,plasmonic nanosensor
更新于2025-09-23 15:21:01
-
Nanoparticle Design Rules for Colorimetric Plasmonic Sensors
摘要： Localized surface plasmon resonance (LSPR) sensors are used in a broad range of detection applications across the chemical, biological, environmental, and medical disciplines. These types of sensors traditionally use the plasmon resonance wavelength of a nanoparticle array to detect changes in refractive index at the sensor surface and therefore require expensive spectroscopic instrumentation for readout. However, simple, portable, and low-cost LSPR sensors can be achieved by transitioning to colorimetric measurements, in which refractive index changes are quantified using the R, G, and B pixel intensities from digital nanoparticle images. In this study we use R, G, and B pixel intensities to quantify color coordinates in the HSV, CIE L*a*b, and rgb chromaticity color spaces. We show that for sensors comprising 115-nm diameter nanoparticles, hue (H) is the most sensitive color parameter, with a change per refractive index unit (Dhue/DRIU) of 0.71 and a figure of merit of 183 RIU-1. Furthermore, we compared hue figures of merit (FOM) for nanoparticles in four different diameters (34.1, 59.8, 81.5, and 115 nm) and showed that hue sensitivity peaks at a diameter of 81.5 nm, with a FOM of 222 RIU-1. In contrast, the spectroscopic sensitivity, quantified in units of Dnm/DRIU, increased continually with nanoparticle size. Therefore, the design requirements for colorimetric plasmonic sensors differ from those for spectroscopic plasmonic sensors. This difference in size dependence was explored further using Mie calculations to simulate nanoparticle extinction spectra. Our results revealed that while lmax responds linearly to refractive index changes, hue responds in a sigmoidal fashion. As a result, the nanoparticle size used in colorimetric sensors relying on hue measurement should be carefully selected to achieve a linear sensor response. We provide general design rules for optimizing hue-based colorimetric sensors and demonstrate that our sensor can be used with a smartphone to detect antibody-antigen interactions.
关键词： portable,refractive index,nanosensor,hue,smartphone,image processing
更新于2025-09-23 15:19:57
-
Dual-Color Plasmonic Nanosensor for Radiation Dosimetry
摘要： Radiation dosimeters are critical for accurately assessing the levels of radiation exposure of tumor sites and surrounding tissues and for optimizing therapeutic interventions as well as for monitoring environmental exposure. To fill the need for a simple, user-friendly and inexpensive dosimeter, we designed an innovative colorimetric nanosensor-based assay for detecting ionizing radiation. We show that hydroxyl radicals generated by ionizing radiation can be used to etch gold nanorods (AuNRs) and silver nanoprisms (AgNPRs), yielding reproducible color changes for radiation dose detection in the range of 50-2000 Rad, broad enough to cover doses used in hyperfractionated, conventional, and hypofractionated radiotherapy. This range of doses detected by this assay correlates with radiation induced DNA damage response in mammalian cells. Furthermore, this AuNR- and AgNPR-based sensing platform has been established in a paper format that can be readily adopted for a wide range of applications and translation.
关键词： Radiation dosimetry,gold nanorods,silver nanoprisms,dual-color nanosensor,colorimetric detection
更新于2025-09-23 15:19:57
-
Nanomaterials based optical and electrochemical sensing of histamine: Progress and perspectives
摘要： Histamine is known to be a principal causative agent associated with marine food poisoning outbreaks worldwide, which is typically formed in the contaminated food by decarboxylation of histidine by bacterial histidine decarboxylase. Upon quantification of histamine in different food products, one can comment on the quality of the food and use it as an indicator of the good manufacturing practices and the state of preservation. The United States Food and Drug Administration (FDA) has established 50 ppm (50 mg/kg) of histamine as the chemical index for fish spoilage. Consumption of foods containing histamine higher than the permissible limit can cause serious health issues. Several methods have been developed for the determination of histamine in a variety of food products. The conventional methods for histamine detection such as thin layer chromatography, capillary zone electrophoresis, gas chromatography, colorimetry, fluorimetry, ion mobility spectrometry, high-performance liquid chromatography, and enzyme-linked immunosorbent assay (ELISA), are being used for sensitive and selective detection of histamine. However, there are a number of disadvantages associated with the conventional techniques, such as multi-step sample processing and requirement of expensive sophisticated instruments, which restrict their applications at laboratory level only. In order to address the limitations associated with the traditional methods, new approaches have been developed by various research groups. Current advances in nanomaterial-based sensing of histamine in different food products have shown significant measurement accuracy due to their high sensitivity, specificity, field deployability, cost and ease of operation. In this review, we have discussed the development of nanomaterials-based histamine sensing assays/strategies where the detection is based on optical (fluorescence, surface enhanced Raman spectroscopy (SERS), localized surface plasmon resonance) and electrochemical (impedimetric, voltammetry, potentiometric, etc.). Further, the advantages, disadvantages and future scope of the nanomaterials-based histamine sensor research are highlighted.
关键词： Optical sensors,Nanosensor,Nanoparticles,Food quality control,Electrochemical sensors,Histamine
更新于2025-09-19 17:15:36