研究目的
To develop a novel SPR nanosensor for selective detection of the anticancer drug etoposide in urine samples using core-shell nanoparticles incorporated with hexagonal boron nitride nanosheets and molecularly imprinted polymers.
研究成果
The developed SPR nanosensor based on Ag@AuNPs-HBN nanocomposite and MIP showed high sensitivity, selectivity, stability, and repeatability for etoposide detection in urine samples, with a low detection limit and good recovery, outperforming other analytical methods.
研究不足
The study may have limitations in terms of the specificity to other complex matrices beyond urine, potential interference from other substances, and the need for further validation in clinical settings. Optimization of the sensor for real-time applications and scalability could be areas for improvement.
1：Experimental Design and Method Selection:
The study involved the development of an SPR-based nanosensor using nanomaterials and molecular imprinting for enhanced sensitivity and selectivity. Methods included SEM, TEM, XRD, CV, EIS, FTIR, and AFM for characterization.
2：Sample Selection and Data Sources:
Urine samples were used for recovery tests, with etoposide and other compounds (e.g., glucose, sucrose) prepared in phosphate buffer solutions.
3：List of Experimental Equipment and Materials:
Equipment included IviumStat for electrochemistry, Rikagu Miniflex X-ray diffractometer, ZEISS EVO 50 SEM, GenOptics SPRi-Lab SPR system. Materials included etoposide, various acids, monomers, and chemicals from Merck.
4：Experimental Procedures and Operational Workflow:
Steps included preparation of HBN nanosheets and Ag@AuNPs-HBN nanocomposite, modification of SPR chip, development of imprinted sensor using spin coating and UV polymerization, ETO removal with NaCl, and analysis using SPR system with adsorption-desorption cycles.
5：Data Analysis Methods:
Data were analyzed using CV and EIS for electrode characterization, and SPR signals were monitored for quantification, with statistical analysis for recovery and selectivity.
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