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Fabrication of gold-silver core-shell nanoparticles for performing as ultrabright SERS-nanotags inside human ovarian cancer cells
摘要： This paper presents the fabrication and characterization of new gold-silver core-shell nanoparticles labeled with para-mercaptobenzoic acid (4MBA) molecules and demonstrates their use as SERS-nanotags with ultra-bright traceability inside cells and ability to convey spectrally-coded information about the intracellular pH by means of Surface-Enhanced Raman Spectroscopy (SERS). Unlike to previous reported studies, our fabrication procedure includes in the first step the synthesis of chitosan-coated gold nanoparticles as a seed material with subsequent growing of a silver shell. The bimetallic core-shell structure is revealed by transmission electron microscopy (TEM), high-angle annular dark field scanning transmission electron microscopy (HAADF-STEM), energy-dispersive X-ray elemental mapping (EDX) and the presence of two interacting localized surface plasmon resonance (LSPR) modes in UV-Vis extinction spectrum. The high SERS activity and sensitivity of as fabricated 4MBA-chit-Au-AgNPs nano-constructs to different pH in solution is investigated under 532 and 633 nm laser lines excitation. Next, in view of future studies in cancer diagnosis, the in vitro antiproliferative effects of SERS-nanotags against human ovarian adenocarcinoma cells (NIH:OVCAR-3) are evaluated. The capacity to operate as bright SERS nanotags with precise localization at a single cell level as well as intracellular pH indicators is clearly demonstrated by performing cell imaging under scanning confocal Raman microscopy.
关键词： core-shell nanoparticles,pH sensors,ovarian cancer cells,chitosan,SERS tags
更新于2025-11-21 11:24:58
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Changes in urine autofluorescence in ovarian cancer patients
摘要： Ovarian cancer is the type of cancer with the highest mortality rate among gynaecologic malignancies. Due to lack of screening tools, this disease is mainly diagnosed at a progressed stage, when it is too late to adequate therapy. Despite many attempts, enough sensitive and specific biomarker was not still uncovered. Fluorescence spectroscopy has proven to be a useful diagnostic tool with high efficiency. Fluorescence detection has three major advantages over other light-based investigation methods: high sensitivity, high speed, and reliability. Biological materials consist of a number of intrinsic fluorescent compounds – autofluorophores, which are associated with cardinal metabolic pathways. It is well known, that cancerous tissue metabolism is altered compared to healthy one, what influence also intrinsic fluorophores composition of bodily fluids. Urine is one of the biological fluids that could be obtained most easily and displays a blue – green fluorescence that can change in case of pathological process. Analysis of urine autofluorescence is non invasive and simple technique. Using fluorescent spectroscopy, ovarian cancer patients and healthy control group were discerned with high significance, so we predict that fluorescence analysis of urine could be a potential means of ovarian cancer screening.
关键词： fluorescence,cancer screening,urine,ovarian cancer
更新于2025-09-23 15:23:52
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Non-Invasive Fluorescent Monitoring of Ovarian Cancer in an Immunocompetent Mouse Model
摘要： Ovarian cancers (OCs) are the most lethal gynaecological malignancy, with high levels of relapse and acquired chemo-resistance. Whilst the tumour–immune nexus controls both cancer progression and regression, the lack of an appropriate system to accurately model tumour stage and immune status has hampered the validation of clinically relevant immunotherapies and therapeutic vaccines to date. To address this need, we stably integrated the near-infrared phytochrome iRFP720 at the ROSA26 genomic locus of ID8 mouse OC cells. Intrabursal ovarian implantation into C57BL/6 mice, followed by regular, non-invasive fluorescence imaging, permitted the direct visualization of tumour mass and distribution over the course of progression. Four distinct phases of tumour growth and dissemination were detectable over time that closely mimicked clinical OC progression. Progression-related changes in immune cells also paralleled typical immune profiles observed in human OCs. Specifically, we observed changes in both the CD8+ T cell effector (Teff):regulatory (Treg) ratio, as well as the dendritic cell (DC)-to-myeloid derived suppressor cell (MDSC) ratio over time across multiple immune cell compartments and in peritoneal ascites. Importantly, iRFP720 expression had no detectible influence over immune profiles. This new model permits non-invasive, longitudinal tumour monitoring whilst preserving host–tumour immune interactions, and allows for the pre-clinical assessment of immune profiles throughout disease progression as well as the direct visualization of therapeutic responses. This simple fluorescence-based approach provides a useful new tool for the validation of novel immuno-therapeutics against OC.
关键词： iRFP720,syngeneic,ovarian cancer,iRFP,tumour,T cell,immune,ID8
更新于2025-09-23 15:22:29
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[IEEE 2019 IEEE International Electron Devices Meeting (IEDM) - San Francisco, CA, USA (2019.12.7-2019.12.11)] 2019 IEEE International Electron Devices Meeting (IEDM) - Multiphysics Simulation & Design of Silicon Quantum Dot Qubit Devices
摘要： In multiclass machine learning problems, one needs to distinguish between the nominal labels that do not have any natural ordering and the ordinal labels that are ordered. Ordinal labels are pervasive in biology, and some examples are given here. In this note, we point out the importance of making use of the order information when it is inherent to the problem. We demonstrate that algorithms that use this additional information outperform the algorithms that do not, on a case study of assigning one of four labels to the ovarian cancer patients on the basis of their time of progression-free survival. As an aside, it is also pointed out that the algorithms that make use of ordering information require fewer data normalizations. This aspect is important in biological applications, where data are plagued by variations in platforms and protocols, batch effects, and so on.
关键词： ovarian cancer,Ordinal classification
更新于2025-09-23 15:19:57
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Photoactivatable Prodrug-Backboned Polymeric Nanoparticles for Efficient Light-Controlled Gene Delivery and Synergistic Treatment of Platinum-Resistant Ovarian Cancer
摘要： Combination of chemotherapy and gene therapy provides an effective strategy for cancer treatment. However, lacking of suitable co-delivery systems with efficient endo/lysosomal escape and controllable drug release/gene unpacking is the major bottleneck for maximizing the combinational therapeutic efficacy. Herein, we developed a photoactivatable Pt(IV) prodrug-backboned polymeric nanoparticles system (CNPPtCP/si(c-fos)) for light-controlled si(c-fos) delivery and synergistic photoactivated chemotherapy (PACT) and RNAi on platinum-resistant ovarian cancer (PROC). Upon blue light irradiation (430 nm), CNPPtCP/si(c-fos) could generate oxygen-independent N3? with mild oxidation energy for efficient endo/lysosomal escape through N3?-assisted photochemical internalization with less gene deactivation. Thereafter, along with Pt(IV) prodrug activation, CNPPtCP/si(c-fos) would be disassociated to release active Pt(II) and unpack si(c-fos) simultaneously. Both in vitro and in vivo results demonstrated that CNPPtCP/si(c-fos) displayed excellent synergistic therapeutic efficacy on PROC with low toxicity. This PACT prodrug-backboned polymeric nanoplatform may provide a promising gene/drug co-delivery tactics for treatment of various hard-to-tackle cancers.
关键词： N3?-assisted photochemical internalization,photoactivatable polymeric nanoparticles,gene delivery,platinum-resistant ovarian cancer,photoactivated chemotherapy
更新于2025-09-19 17:13:59
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A Nanosystem Loaded Perfluorohexane and Rose Bengal Coupled Upconversion Nanoparticles for Multimodal Imaging and Synergetic Chemo-Photodynamic Therapy in Cancer
摘要： Theranostics is a new trend integrating diagnostic and therapeutic functions in tumour research. Theranostic nanoparticles that enabling both tumour imaging and drug delivery are a promising platform for image-guided cancer therapy. Photodynamic therapy (PDT) has great potential in synergy with traditional chemotherapy while facing great challenges due to hypoxia, poor targeting ability and limited penetration depth of visible light. To solve these problems, we presented a novel nanosystem of FA/UCNPs-RB/HCPT/PFH@Lipid (denoted as FURH-PFH-NPs), with a perfluorohexane (PFH) carrying rich oxygen core and a folic acid-modified lipid shell. The shell contains 10-hydroxycamptothecin (HCPT) and self-fluorescing photosensitizer compounds, namely, of upconversion nanoparticles and Rose Bengal (UCNPs-RB). In this study, FURH-PFH-NPs aggregated at SKOV3 cells (in vitro) and the nude xenograft tumour region when combined with folic acid receptor. When triggered by low-intensity focused ultrasound (LIFU), FURH-PFH-NPs released PFH, UCNPs-RB and HCPT. The above procedure was monitored through multimodal imaging, which simultaneously guided the tumour therapy. UCNPs-RB and PFH promoted the PDT effect under LIFU. Through PDT and HCPT, we obtained better therapeutic effects and good biosafety in SKOV3 nude xenograft tumours. FURH-PFH-NPs combined with LIFU and laser irradiation might be a promising strategy for ovarian cancer.
关键词： Photodynamic therapy,Theranostics,ovarian cancer,multimodal imaging,nanoparticles
更新于2025-09-19 17:13:59
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Development of Highly Reliable SERS‐active Photonic Crystal Fiber Probe and its Application in the Detection of Ovarian Cancer Biomarker in Cyst Fluid
摘要： Conventionally Surface enhanced Raman spectroscopy (SERS) is realized by adsorbing analytes onto nano-roughened planar substrate coated with noble metals (silver or gold) or their colloidal nanoparticles (NPs). Nano scale irregularities in such substrates/NPs could lead to SERS sensors with poor reproducibility and repeatability. Herein, we demonstrate a suspended core photonic crystal fiber (PCF) based SERS sensor with extremely high reproducibility and repeatability in measurement with a relative standard deviation of only 1.5% and 4.6% respectively, which makes it more reliable than any existing SERS sensor platforms. In addition, our platform could improve the detection sensitivity owing to the increased interaction area between the guided light and the analyte, which is incorporated into the holes that runs along the length of the PCF. Numerical calculation established the significance of the interplay between light coupling efficiency and evanescent field distribution, which could eventually determine the sensitivity and reliability of the developed SERS active-PCF sensor. As a proof of concept, using this sensor, we demonstrated the detection of haptoglobin, a biomarker for ovarian cancer, contained within the ovarian cyst fluid, which facilitated in differentiating the stages of the cancer. We envision that with necessary refinements, this platform could potentially be translated as a next generation highly sensitive SERS-active opto-fluidic biopsy needle for the detection of biomarkers in body fluids.
关键词： reproducibility and repeatability in measurement,Surface enhanced Raman scattering,photonic crystal fiber,biopsy needle,protein sensing,ovarian cancer
更新于2025-09-12 10:27:22
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<p>The Destruction Of Laser-Induced Phase-Transition Nanoparticles Triggered By Low-Intensity Ultrasound: An Innovative Modality To Enhance The Immunological Treatment Of Ovarian Cancer Cells</p>
摘要： Photodynamic therapy (PDT), sonodynamic therapy (SDT), and oxaliplatin (OXP) can induce immunogenic cell death (ICD) following damage-associated molecular patterns (DAMPs) exposure or release and can be united via the use of nanoplatforms to deliver drugs that can impart anti-tumor effects. The aim of this study was to develop phase-transition nanoparticles (OI_NPs) loaded with perfluoropentane (PFP), indocyanine green (ICG), and oxaliplatin (OXP), to augment anti-tumor efficacy and the immunological effects of chemotherapy, photodynamic therapy and sonodynamic therapy (PSDT).
关键词： photo-sonodynamic therapy,multifunctional nanoparticles,immunogenic cell death,reactive oxygen species,ovarian cancer
更新于2025-09-12 10:27:22
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Electrochemiluminescence Resonance Energy Transfer between Ru(bpy) <sub/>3</sub><sup>2+</sup> and CdZnSe@ZnSe Quantum Dots for Ovarian Cancer Biomarker Detection
摘要： Herein, an enhanced electrochemiluminescence resonance energy transfer (ECL-RET) from Ru(bpy)3 2+ to the core/shell CdZnSe@ZnSe quantum dots (CdZnSe@ZnSe QDs) was first designed for ovarian cancer biomarker analysis. The TiO2 metal-organic frameworks (TiO2 MOFs) was used as promoter because of its unique semiconductor structure and high loading ability for Ru(bpy)3 2+. Additionally, Envision complex with numerous horseradish peroxidase (HRP) was employed to immobilize CdZnSe@ZnSe QDs, the acceptor of ECL-RET, which further improved the ECL emission of CdZnSe@ZnSe QDs. Concretely, reactive oxygen species (ROS), for instance, O2 ?? and OH?, were firstly yielded due to the catalytic ability of HRP to H2O2, and then the electron from O2 ?? or hole from OH? injection into the CdZnSe@ZnSe QDs, triggering an extremely strong ECL response of CdZnSe@ZnSe QDs. On the basis of all above features, a highly effective ECL-RET biosensor was elaborately established to detect the targets in the range of 1.00×10-6?1.00×102 ng/mL with low detection limit of 3.30×10-1 fg/mL (S/N=3). This work opened up a new avenue for developing high-performance ECL-RET biosensors and demonstrated the high potential of the new biomarker in clinical ovarian cancer screening.
关键词： CdZnSe@ZnSe QDs,TiO2 MOFs,Envision complex,ECL-RET,ovarian cancer biomarker
更新于2025-09-12 10:27:22
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New 55Co-labeled Albumin-Binding Folate Derivatives as Potential PET Agents for Folate Receptor Imaging
摘要： Overexpression of folate receptors (FRs) on di?erent tumor types (e.g., ovarian, lung) make FRs attractive in vivo targets for directed diagnostic/therapeutic agents. Currently, no diagnostic agent suitable for positron emission tomography (PET) has been adopted for clinical FR imaging. In this work, two 55Co-labeled albumin-binding folate derivatives-[55Co]Co-cm10 and [55Co]Co-rf42-with characteristics suitable for PET imaging have been developed and evaluated. High radiochemical yields (≥95%) and in vitro stabilities (≥93%) were achieved for both compounds, and cell assays demonstrated FR-mediated uptake. Both 55Co-labeled folate conjugates demonstrated high tumor uptake of 17% injected activity per gram of tissue (IA/g) at 4 h in biodistribution studies performed in KB tumor-bearing mice. Renal uptake was similar to other albumin-binding folate derivatives, and liver uptake was lower than that of previously reported [64Cu]Cu-rf42. Small animal PET/CT images con?rmed the biodistribution results and showed the clear delineation of FR-expressing tumors.
关键词： folate receptors,cobalt-55,PET imaging,albumin binder,ovarian cancer,folic acid
更新于2025-09-12 10:27:22