- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Effectiveness of Adjunctive Use of Low-Level Laser Therapy and Photodynamic Therapy After Scaling and Root Planing in Patients with Chronic Periodontitis
摘要： The aim of this split-mouth, randomized controlled clinical trial was to evaluate the efficacy of low-level laser therapy (LLLT) and photodynamic therapy (PDT) as an adjunct to scaling and root planing (SRP) in treatment of chronic periodontitis. Each quadrant was categorized into control group (SRP alone; two quadrants per patient), test group 1 (SRP + PDT), and test group 2 (SRP + LLLT. The test groups showed significantly higher reductions in Gingival Index, probing depth, and clinical attachment level as well as reductions in Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans counts at 1-, 3-, 6-, and 9-month follow-ups when compared with the control group.
关键词： photodynamic therapy,chronic periodontitis,low-level laser therapy,scaling and root planing
更新于2025-09-11 14:15:04
-
Synergistic Nanozymetic Activity of Hybrid Gold Bipyramid- Molybdenum Disulfide Core@Shell Nanostructures for Two-Photon Imaging and Anticancer Therapy
摘要： In recent years, the concept of combined therapy using gold hybrid nanomaterials has been broadly adopted to pioneer new anticancer treatments. However, their synergistic anticancer effects have yet to be thoroughly investigated. Herein, a hybrid gold nanobipyramid nanostructure coated with molybdenum disulfide (MoS2) semiconductor (AuNBPs@MoS2) was proposed as a smart nanozyme for anticancer therapy and two-photon bioimaging. The hybrid material showed dramatically enhanced localized surface plasmon resonance (LSPR) property under excitation owing to its anisotropic nature, coupled with the rich electron density in MoS2, resulting in the superior in situ photo-generation of reactive oxidative species (ROS - 1O2, ˙OH). We demonstrated that the synergistic effect of enhanced photothermal conversion and generation of ROS could increase the anticancer effect of AuNBPs@MoS2. Two-photon luminescence (TPL) imaging confirmed that AuNBPs@MoS2 was successfully internalized in cancer cells and that simultaneous anticancer treatments based on catalytic and photothermal therapy could be achieved. This study highlighted, for the first time, a novel approach of plasmon-mediated powerful anticancer therapy and imaging via the unprecedented combination of anisotropic AuNBPs and 2D MoS2 material.
关键词： localized surface plasmon resonance,photothermal therapy,photodynamic therapy,gold nanobipyramid,two-photon imaging
更新于2025-09-11 14:15:04
-
Transfoveal Micropulse Laser Treatment of Central Serous Chorioretinopathy within Six Months of Disease Onset
摘要： Background: It has been recommended that any invasive treatment performed in patients with central serous chorioretinopathy (CSCR) not be initiated earlier than four months after disease onset due to the potential for spontaneous remission of symptoms. The goal of this study was to examine the outcome of transfoveal subthreshold micropulse laser treatment (SMPLT) of CSCR performed at six months or less after disease onset. Materials and methods: The study included 32 cases of CSCR lasting between three weeks and six months (mean: 3.4 ± 2.3 months). All patients had transfoveal SMPLT applied and were followed for at least three months after each session of SMPLT. Two sessions of SMPLT in total were planned in case of an insu?cient response to the ?rst instance of treatment. Evaluation parameters included any change in best-corrected visual acuity (BCVA) and retinal morphology. Results: Total resolution of subretinal ?uid (SRF) was noted in 26 cases (81.25%). Final BCVA improved signi?cantly from 0.37 ± 0.22 logMAR to 0.22 ± 0.20 logMAR after treatment. Overall, early SMPLT correlated with better ?nal BCVA (p = 0.0005, Spearman rank correlation). For eyes achieving a total resolution of SRF, BCVA improved from 0.33 ± 0.21 logMAR to 0.17 ± 0.14 logMAR (p = 0.004, Spearman rank correlation). The analysis of SMPLT nonresponders revealed a tendency for poorer baseline visual acuity. Conclusions: Patients with CSCR lasting six months or less treated with transfoveal SMPLT achieve better functional results with early application of this procedure. As baseline BCVA predicts ?nal visual acuity, earlier treatment, permitted by the safety of SMPLT, may improve ?nal visual outcomes.
关键词： central serous chorioretinopathy,photodynamic therapy,subthreshold micropulse laser,subretinal ?uid
更新于2025-09-11 14:15:04
-
Effect and mechanism of ZrO <sub/>2</sub> doping on the cracking behavior of melt‐grown Al <sub/>2</sub> O <sub/>3</sub> ceramics prepared by directed laser deposition
摘要： Photodynamic therapy (PDT), a treatment of choice for cancer, induces a photochemical reaction, thereby eradicating tumor cells. This is achieved through the administration of a photosensitizer drug, which is activated with a laser after localization to the tumor mass, and is an approved curative endoscopic ablative treatment for superficial esophageal squamous cell carcinoma (ESCC) in Japan. PDT has been approved for dysplastic Barrett’s esophagus and as a palliative treatment for patients with symptomatic obstructive esophageal cancer in US. However, its adverse events and complicated procedure and the development of alternative endoscopic procedures such as endoscopic submucosal dissection, radiofrequency ablation and cryotherapy, have largely limited the practice of PDT in esophageal cancer worldwide. Recently, owing to the invention of second-generation PDT using talaporfin sodium and diode laser, PDT can be performed with less phototoxicity and therefore has regained popularity in the treatment of ESCC. As a salvage treatment for patients with local failure after chemoradiotherapy (CRT), PDT has shown promising complete response with less phototoxicity and shorter sun shade period. In addition, the efficacy and safety of PDT in patients with local failure of ESCC after CRT were shown in several clinical trials. The direction of the study interest of the next-generation PDT is the safety and potential expansion of the indications for its application in the future. This review covers the PDT for the treatment of ESCC and dysplastic Barrett’s esophagus, with special focus on the role of PDT in practice for esophageal cancer.
关键词： photodynamic therapy,endoscopic submucosal dissection,esophageal cancer,squamous cell carcinomas,endoscopic treatment
更新于2025-09-11 14:15:04
-
Ablative fractional laser‐assisted photodynamic therapy for lentigo maligna: a prospective pilot study
摘要： Background Lentigo maligna (LM) is an in situ form of melanoma carrying a risk of progression to invasive lentigo maligna melanoma (LMM). LM poses a clinical challenge, with subclinical extension and high recurrence rates after incomplete surgery. Alternative treatment methods have been investigated with varying results. Photodynamic therapy (PDT) with methylaminolaevulinate (MAL) has already proved promising in this respect. Objectives To investigate the efficacy of ablative fractional laser (AFL)-assisted PDT with 5-aminolaevulinic acid nanoemulsion (BF-200 ALA) for treating LM. Methods In this non-sponsored prospective pilot study, ten histologically verified LMs were treated with AFL-assisted PDT three times at 2-week intervals using a light dose of 90 J/cm2 per treatment session. Local anaesthesia with ropivacaine was used. Four weeks after the last PDT treatment the lesions were treated surgically with a wide excision and sent for histopathological examination. The primary outcome was complete histopathological clearance of the LM from the surgical specimen. Patient-reported pain during illumination and the severity of the skin reaction after the PDT treatments were monitored as secondary outcomes. Results The complete histopathological clearance rate was 7 out of 10 LMs (70%). The pain during illumination was tolerable, with the mean pain scores for the PDT sessions on a visual assessment scale ranging from 2.9 to 3.8. Some severe skin reactions occurred during the treatment period, however. Conclusions Ablative fractional laser-assisted PDT showed moderate efficacy in terms of histological clearance. It could constitute an alternative treatment for LM but due to the side effects it should only be considered in inoperable cases.
关键词： Histopathological clearance,5-aminolaevulinic acid,Ablative fractional laser,Photodynamic therapy,Lentigo maligna
更新于2025-09-11 14:15:04
-
Photodynamic therapy in 2D and 3D human cervical carcinoma cell cultures employing LED light sources emitting at different wavelengths
摘要： Light of different wavelengths can be used to obtain a more profitable outcome of photodynamic therapy (PDT), according to the absorption bands of the photosensitizer (PS). Low-grade cervical intraepithelial neoplasias (CINs) are superficial lesions that can be treated with light of shorter wavelength than red because a large light penetration depth in tissue is not necessary. We report a comparative investigation performed to evaluate the efficacy of light-emitting diodes (LEDs) of different wavelengths in the photodynamic treatment applied to both 2D and 3D HeLa cell spheroid cultures. The spheroids are utilized as a PDT dosage model, and cell viability is evaluated at different sections of the spheroids by confocal microscopy. Cells incubated with m-tetrahydroxyphenyl chlorin are illuminated with LED systems working in the low fluence range, emitting in the violet (390 – 415 nm), blue (440-470 nm), red (620 – 645 nm) and deep red (640 – 670 nm) regions of the light spectrum at various exposures times (tI) comprised between 0.5 and 30 min. PDT experiments performed on both 2D and 3D cell cultures indicate that the PDT treatment outcome is more efficient with violet light followed by red light. Dynamic data from the front displacement velocity of large 2D-quasi-radial colonies generated from cell spheroids adhered to the Petri dish bottom as well as the evolution of the 3D growth give further insight about the effect of PDT at each condition. Results from 3D cultures indicate that the penetration of the violet light is appropriate to kill HeLa cells several layers below, showing cell damage and death not only in the outer rim of the illuminated spheroids, where a PS accumulation exists, but also in the more internal region. Results indicate that violet LED light could be useful to treat CINs involving superficial dysplasia.
关键词： LEDs,cervical intraepithelial neoplasias,Photodynamic therapy,m-THPC,2D and 3D HeLa cell culture
更新于2025-09-11 14:15:04
-
Acne treatment efficacy of intense pulsed light photodynamic therapy with topical licochalcone A, <scp>l</scp> ‐carnitine, and decanediol: A spilt‐face, double‐blind, randomized controlled trial
摘要： Background: Depending on disease severity, standard acne treatments can vary from topical to systemic therapy. However, poor compliance caused by adverse events and antibiotic resistance is a major cause of treatment failure. Aims: To determine the effectiveness of photodynamic therapy (PDT) with intense pulsed light (IPL) in the treatment of acne when combined with a cream containing licochalcone A, L‐carnitine and decanediol (so‐called, ‘active formulation’) versus PDT alone. Patients/Methods: Twenty‐nine volunteers, aged 21‐39 years (26 women and 3 men, mean age 29.41 ± 5.24 years), with mild to severe facial acne, were enrolled. Each subject’s face sides were randomized in a split‐face manner to either receive PDT (IPL with a 400‐720 nm cut‐off filter, at 4 sessions with two‐week intervals) combined with the active formulation cream twice daily for 10 weeks on one face side; or PDT and the vehicle cream on the other side, with the same treatment protocol. Reduction in acne quantity, melanin index and erythema index were assessed 2 weeks after the second treatment (day 28), 1 week after the fourth treatment (day 49), and 1 month after the fourth treatment (day 70). Results: Compared to baseline, patients in the active formulation group demonstrated a faster onset of reduction in the number of lesions at 2 weeks after the second treatment (p=0.010 for inflammatory acne and p=0.001 for non‐inflammatory acne). A significantly greater reduction in lesion count was observed in the active formulation group compared with the vehicle group at all timepoints of evaluation for noninflammatory acne (day 28, day 49, and day 70; p=0.003, 0.005 and 0.002 respectively), and at 1 month after the fourth treatment for inflammatory acne (p=0.036). Compared to the vehicle group, the melanin index of the active formulation group decreased significantly at 1 month after the fourth treatment (p=0.015). Conclusion: PDT is more effective in treating acne when combined with a topical cream containing licochalcone A, L‐carnitine and decanediol, than PDT alone. Significant acne reduction and improvements in post‐inflammatory hyperpigmentation were observed, which offers acne patients a better therapeutic option. It is a safe and effective combination treatment for patients with moderate and severe acne.
关键词： photodynamic therapy,licochalcone A,l‐carnitine,acne,decanediol,intense pulsed light
更新于2025-09-11 14:15:04
-
Triplet Excited State Enhancement Induced by PDDA Polymer Assembled Gold Nanoparticles
摘要： Gold nanoparticles (AuNPs) have unique optical properties due to their characteristic localized surface plasmon resonances (LSPR). Upon the excitation of LSPR, AuNPs can affect the adjacent organic molecules strongly in both the ground state and the excited state. Previously, only 2~3 fold of plasmon enhancement effect on triplet state formation was displayed due to the random and uncontrollable formation of AuNP aggregates. Here, we utilize polymer PDDA to assemble AuNPs forming aggregates with strong plasmonic resonance. By means of transient UV-visible absorption spectroscopy, the triplet state of rose bengal enhanced by PDDA-AuNPs is directly monitored and the maximum enhancement is found to be ~ 10 fold. The large enhancement should be mainly resulted from the plasmon effect of AuNPs aggregates. Additionally, it is found that the triplet state enhancement effect of PDDA assembled AuNPs is sensitive to concentration of polymer and the size of AuNPs. These findings shed light on the applications of gold nanoparticles in triplet-triplet energy transfer, triplet exciton harvesting and photodynamic therapy.
关键词： PDDA polymer,Gold nanoparticles,Plasmon enhancement,Triplet excited state,Photodynamic therapy
更新于2025-09-11 14:15:04
-
Phase‐Change Materials Based Nanoparticles for Controlled Hypoxia Modulation and Enhanced Phototherapy
摘要： Tumor hypoxia strengthens tumor resistance to different therapies especially oxygen involved strategies, such as photodynamic therapy (PDT). Herein, the thermal responsive phase change materials (PCM) are utilized to coencapsulate ultrasmall manganese dioxide (sMnO2) and organic photosensitizer IR780 to obtain IR780-sMnO2-PCM nanoparticles for controlled tumor hypoxia modulation and enhanced phototherapy. The thermal responsive protective PCM layer can not only prevent IR780 from photodegradation, but also immediately release sMnO2 to decompose endogenous H2O2 and generate enough oxygen for PDT under laser irradiation. Owing to the efficient accumulation of IR780-sMnO2-PCM nanoparticles in tumor under intravenous injection as revealed by both florescence imaging and photoacoustic imaging, the tumor hypoxia is greatly relieved. Furthermore, in vivo combined photothermal therapy (PTT) and PDT, IR780-sMnO2-PCM nanoparticles, compared to IR780-PCM nanoparticles, exhibit better performance in inhibiting tumor growth. The results highlight the promise of IR780-sMnO2-PCM in controlled modulation of tumor hypoxia to overcome current limitations of cancer therapies.
关键词： hypoxia modulation,cancer theranostics,photodynamic therapy,phase change materials
更新于2025-09-11 14:15:04
-
Efficacy of Antimicrobial Photodynamic Therapy as an Adjunctive to Mechanical Debridement in the Treatment of Peri-implant Diseases: A Randomized Controlled Clinical Trial
摘要： Introduction: The purpose of the present study was to assess the clinical effects of antimicrobial photodynamic therapy (PDT) after closed surface scaling in the treatment of peri-implant diseases. Methods: Ten patients with a total of 15 pairs of dental implants, showing clinical and radiographic signs of peri-implant diseases, were included in this study. In each patient, one implant randomly served as control implant and the other served as test implant. The control implants were treated with closed surface scaling only and the test implants received additionally PDT, using light with a wavelength of 630 nm and intensity of 2000 mw/cm2 for 120 seconds after application of photosensitizer in peri-implant sulcus. Clinical parameters were evaluated before and 1.5 and 3 months after treatment. Results: Statistical analysis showed significant differences in probing pocket depth (PPD), clinical attachment loss (CAL), bleeding on probing (BOP), and gingival index (GI) at each time point between the two groups. There were no statistically significant changes with respect to any of the parameters in the control group. Complete resolution of BOP at 3 months was achieved in 100% of test implants. At 1.5 and 3 months, there were significant differences in the mean probing depth and CAL gain measurements at implants in the test group. Conclusion: The present study revealed that adjunctive use of PDT following closed surface scaling could lead to clinical improvement of peri-implant diseases. Further studies are necessary to confirm our results.
关键词： photodynamic therapy,mechanical debridement,peri-implantitis,dental implant,clinical trial
更新于2025-09-11 14:15:04