研究目的
Investigating the effectiveness of methylene blue with sodium bicarbonate on Candida albicans sterilization using photodynamic antimicrobial chemotherapy.
研究成果
PACT using basic MB was effective for C. albicans sterilization, suggesting a promising, cost-effective, and simple alternative to conventional treatment for superficial candidiasis.
研究不足
The study was conducted under in vitro conditions, and the effectiveness may vary in clinical settings. The optimal conditions for the basic effect were narrow (pH 8.6-8.9, NaHCO3 concentration 1%-2%, energy fluencies 10-15 J/cm2).
1：Experimental Design and Method Selection:
The study utilized photodynamic antimicrobial chemotherapy (PACT) with methylene blue (MB) and sodium bicarbonate (NaHCO3) under basic pH conditions, followed by irradiation using a red LED.
2：Sample Selection and Data Sources:
C. albicans strain JCM No.1537 was used, identified using the API20 CAUX kit.
3：List of Experimental Equipment and Materials:
MB, NaHCO3, Sabouraud agar medium, red LED (660 nm wavelength), and sterile equipment were used.
4：Experimental Procedures and Operational Workflow:
MB and NaHCO3 concentrations were adjusted, C. albicans was smeared on a medium, irradiated at various energy fluencies, and cultured for 48 h.
5：Data Analysis Methods:
Fungicidal effect was evaluated based on fungal growth area, categorized into six grades.
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