研究目的
To study the photobleaching effect of laser radiation in the range of 210-290 nm on biological tissue spectra.
研究成果
The photobleaching influence on excitation-emission matrices of biological tissue had been investigated. The slowest photobleaching rate occurs at 250 nm wavelength, while faster photobleaching occurs in the wavelengths range of 210-230 nm. It was found that during the standard measurement of the excitation-emission matrix the fluorescence intensity of biological samples drops up to 3.4% in 210–230 nm wavelength range. However, this fact has little effect on the spectra at longer wavelengths and, therefore, the shape of the matrices due to the small depth of radiation penetration at these wavelengths.
研究不足
The study is limited to rat brain tissues and the wavelength range of 210-290 nm. The effect of photobleaching on other types of biological tissues and at other wavelengths was not investigated.
1：Experimental Design and Method Selection:
The study used a tunable laser for fluorescence excitation in the range of 210–290 nm with a step of 10 nm. The fluorescence spectra were measured depending on irradiation dose and wavelength.
2：Sample Selection and Data Sources:
Rat brain tissues were used as the object of study due to the simplicity of their fluorescence spectra.
3：List of Experimental Equipment and Materials:
Pulse-periodic laser Vibrant HE 355 II + UV (Opotek, USA) with optical parametric oscillator, Action SP2300 Spectrometer (Princeton Instruments, USA), photodiode FD-
4：Experimental Procedures and Operational Workflow:
Photobleaching was performed until the intensity of fluorescence at wavelength 322 nm falls at least 3 times. The differences between samples’ excitation spectra before and after photobleaching were investigated.
5：Data Analysis Methods:
The photobleaching rate was estimated as an absorbed radiation dose when peak fluorescence intensity decreases 2.7 times.
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