研究目的
Investigating the therapeutic effects of multifunctional carbon dot-decorated Ag/Au bimetallic nanoshells (CAANSs) as smart plasmonic nanozymes for combined chemo-photothermal cancer therapy and revealing the tryptophan metabolic apoptotic pathway.
研究成果
The study demonstrates that CAANSs act as effective plasmonic nanozymes for cancer therapy by catalyzing the conversion of H2O2 to cytotoxic ROS, revealing a novel tryptophan metabolic pathway in cell apoptosis. This approach offers a promising platform for cancer therapeutics and molecular biology studies.
研究不足
The study is limited by the specificity of the nanozymes to the tumor microenvironment and the potential for off-target effects in normal cells. Further optimization is needed for clinical translation.
1：Experimental Design and Method Selection:
The study involved the synthesis of CAANSs and their application in cancer therapy. The methodology included the use of plasmonic nanozymes for catalytic conversion of H2O2 to ROS under mild acidic conditions.
2：Sample Selection and Data Sources:
HeLa cells were used as the model system. Data were collected through various spectroscopic and imaging techniques.
3：List of Experimental Equipment and Materials:
Included TEM for characterization, UV-vis spectroscopy for catalytic activity assays, and fluorescence microscopy for cell imaging.
4：Experimental Procedures and Operational Workflow:
Detailed steps included the preparation of CAANSs, catalytic activity assays, and in vitro and in vivo therapeutic evaluations.
5：Data Analysis Methods:
Included Michaelis-Menten kinetics for enzyme activity, ESR for radical detection, and SERS for molecular event monitoring.
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