研究目的
To develop a noncontact photoacoustic imaging (PAI) system using an air-coupled ultrasonic transducer for the evaluation of burn injury by discriminating viable and thermally coagulated blood in vivo.
研究成果
The noncontact PAI system equipped with an air-coupled ultrasonic transducer effectively discriminates viable from thermally coagulated blood in vivo, offering a promising tool for the adjuvant diagnosis and observation of burns. Future improvements include developing a higher frequency transducer and incorporating multi-wavelength applications for greater imaging depth and clinical utility.
研究不足
The current system requires a higher frequency air-coupled focused transducer for improved axial resolution and sensitivity. Additionally, multi-wavelength applications and PA blood flow measurement methods could enhance clinical applications.
1:Experimental Design and Method Selection:
The study utilized a noncontact PAI system with a custom-designed air-coupled ultrasonic transducer to image vasculature without contrast agents. The system leveraged the natural difference in light absorption between coagulated and non-coagulated blood at 532 nm.
2:Sample Selection and Data Sources:
Phantom experiments and in vivo imaging of burned rabbit’s skin were conducted to validate the system's capability.
3:List of Experimental Equipment and Materials:
A Q-switched pulsed laser, air-coupled ultrasonic transducer, spatial filter, fiber coupler, beam collimator, plan achromat objective, two-dimensional scanner, low noise amplifier, data acquisition card, and a computer for image reconstruction were used.
4:Experimental Procedures and Operational Workflow:
The laser beam was directed through a spatial filter and coupled into a single-mode fiber. The imaging probe scanned the sample, and PA signals were amplified, digitized, and stored for reconstruction.
5:Data Analysis Methods:
The PA signals were analyzed to distinguish between viable and thermally coagulated blood based on signal intensity differences.
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Fiber coupler
PAF-X-7-A
Thorlabs
Coupling the laser beam into a single mode fiber
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Beam collimator
F240FC-532
Thorlabs
Collimating the laser beam
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Plan achromat objective
RMS4
Thorlabs
Focusing the laser beam
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Impedance analyzer
4294A
Agilent
Measuring electrical impedances of the piezocomposite disk and the final transducer
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Pulser/receiver
5073PR
Olympus
Driving the transducer to emit ultrasonic waves
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Q-switched pulsed laser
DTL314
Laser-export
Excitation light source for photoacoustic imaging
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Low noise amplifier
AU-1291
MITEQ
Amplifying the collected PA signals
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Data acquisition card
M3i.4110
SPECTRUM
Digitizing the amplified PA signals
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Hydrophone
HPM02
Precision Acoustics Ltd.
Detecting ultrasonic waves from the transducer
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