研究目的
To develop ratiometric fluorescence sensors based on CdSe/ZnS quantum dots for the detection of hydrogen peroxide.
研究成果
CdSe/ZnS QDs and aminofluorescein (AF)-encapsulated melamine-formaldehyde particles were successfully employed to fabricate a ratiometric fluorescence membrane for the detection of hydrogen peroxide. The HRP–QD–AF membrane showed higher sensitivity than the QD–AF membrane, although both H2O2-sensing membranes had good selectivity. The HRP–QD–AF membrane could be applied to determine the concentration of hydrogen peroxide in wastewater, while the QD–AF membrane could be used as a new analytical method to detect α-ketobutyrate at low and high concentrations.
研究不足
The QD–AF membrane showed its limitation because its lifetime was not very long in terms of sensitivity at high concentrations of H2O2. The sensitivity of the HRP–QD–AF membrane changed significantly when the temperature was over 33°C for H2O2 in the high concentration range.
1：Experimental Design and Method Selection:
The study involved the synthesis of carboxyl group functionalized-CdSe/ZnS quantum dots (QDs) and aminofluorescein (AF)-encapsulated polymer particles, which were immobilized to a sol–gel mixture for the fabrication of a hydrogen peroxide-sensing membrane. CdSe/ZnS QDs were used for the redox reaction of hydrogen peroxide (H2O2) via a reductive pathway, while AF was used as a reference dye.
2：Sample Selection and Data Sources:
The samples used included CdSe/ZnS QDs and AF-encapsulated polymer particles. Data was collected from the fluorescence intensity of the H2O2-sensing membrane at two emission wavelengths.
3：List of Experimental Equipment and Materials:
Materials included cadmium acetate, selenium, zinc acetate, hexamethyldisilathiane, trioctylphosphin oxide, trioctylphosphine, ethyl cellulose, mercaptopropionic acid, 4-dimethylamino pyridine, 1-hexadecylamine, stearic acid, 3-glycidoxypropyl trimethoxysilane, 3-aminopropyltrimethoxysilane, aminofluorescein, melamine, paraformaldehyde, horseradish peroxidase, hydrogen peroxide, and sodium α-ketobutyrate.
4：Experimental Procedures and Operational Workflow:
The synthesis of CdSe/ZnS QDs and AF-encapsulated MF particles, preparation of H2O2-sensing membranes, and fluorescence measurements were conducted.
5：Data Analysis Methods:
The ratiometric calculation for the H2O2-sensing membrane was based on the ratio of fluorescence intensities at two emission wavelengths. Differences in the ratiometric fluorescence intensity were assessed by one-way analysis of variance (ANOVA).
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
