研究目的
To increase the depth of field (DOF) for in vivo tissue imaging by combining laser speckle contrast imaging (LSCI) with light field (LF) microscopy.
研究成果
The DOF is successfully extended in LSCI with the help of LF. This system will be a powerful tool in hemodynamic monitoring, with potential applications in inflammation, shock, frostbite, and burn assessment.
研究不足
The spatial resolution of the final reconstructed images is not as good as conventional LSCI due to the intrinsic principles of light field imaging. Resolution enhancement can be achieved by multiple methods, but this may require additional processing.
1：Experimental Design and Method Selection:
A modified light field microscope imaging system is designed based on a commercially available LF camera with a microscope lens and a relay lens added to increase the magnification and the field of view.
2：Sample Selection and Data Sources:
Hollow glass tubes immersed in scattering gel are used to simulate the vessels in living tissue. Dairy cream flowing inside the hollow glass tubes mimic blood flow.
3：List of Experimental Equipment and Materials:
He-Ne laser, microscope objective lens, relay lens, LF camera (Lytro illum), scattering gel (1% agarose, 2% dairy cream), hollow glass tubes.
4：Experimental Procedures and Operational Workflow:
The imaging system is adjusted to focus on the sample, and LF images are captured. The DOF is extended using algorithms, and flow maps are generated using LSTCA method.
5：Data Analysis Methods:
Speckle temporal contrast image is constructed by calculating the speckle temporal contrast of each image pixel in the time sequence.
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Lytro illum
Lytro
Capturing light field images
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Microscope objective lens
M plan Apo L; 10×; NA=0.28; f=20 mm
Magnifying the interested area
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Relay lens
f = 40 mm
Increasing the field of view
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He-Ne laser
Light source
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Scattering gel
1% agarose, 2% dairy cream
Mimicking scattering tissue
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Hollow glass tubes
Outer diameter of 500 μm and inner diameter of 300 μm
Simulating the vessels in living tissue
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Syringe pump
Enforcing a controlled blood flow
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