研究目的
To monitor skin S. aureus colonization on AK lesions using FTIR spectroscopic techniques.
研究成果
The cross-lab study demonstrates the applicability of Canadian isolate-based ATR- and transflection-FTIR spectral databases for the identification of clinical staphylococcal isolates obtained in Australia. The results support the potential utility of FTIR spectroscopic techniques to monitor skin S. aureus colonization on AK lesions.
研究不足
The study is limited by the sample size and the specificity of the spectral databases used.
1：Experimental Design and Method Selection:
FTIR spectra of staphylococcal isolates were acquired by both attenuated total reflectance (ATR)-FTIR and transflection-FTIR spectroscopy.
2：Sample Selection and Data Sources:
51 staphylococcal isolates from AK, SCC and perilesional skin were used, previously characterized by 16S rRNA sequencing.
3：List of Experimental Equipment and Materials:
ATR- and transflection-FTIR spectroscopy equipment.
4：Experimental Procedures and Operational Workflow:
Spectra were recorded in triplicate from isolated colonies taken from the same agar plate.
5：Data Analysis Methods:
Identification was based on the similarities of their spectra with those in ATR- and transflection-FTIR spectral databases.
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