研究目的
To develop a novel dopamine enveloped tungsten oxide nanodots (WO3?x/Dpa-Mel NPs) for synergistic photothermal/photodynamic therapy against cancer, activated by a single 808 nm NIR laser, to avoid the overheating effect associated with 980 nm laser and to achieve deep tissue penetration.
研究成果
The WO3?x/Dpa-Mel NPs synthesized via a facile one-step solvothermal route exhibit excellent photothermal and photodynamic properties under single 808 nm laser irradiation, enabling synergistic PTT and PDT against cancer. The NPs show good biocompatibility and dispersity in aqueous solution, making them suitable for in vivo applications. The study demonstrates the potential of WO3?x/Dpa-Mel NPs as a multifunctional therapeutic nanoplatform for cancer therapy, with significant promise for future development in the field.
研究不足
The study focuses on the synthesis and preliminary in vitro and in vivo evaluation of WO3?x/Dpa-Mel NPs. Further research is needed to optimize the synthesis process, enhance the photothermal and photodynamic efficiency, and evaluate the long-term biocompatibility and therapeutic efficacy in more complex cancer models.
1：Experimental Design and Method Selection:
The WO3?x/Dpa-Mel NPs were synthesized via a one-pot solvothermal route using dopamine as the template and reductant in triethylene glycol solvent. The methodology aimed to achieve simultaneous PTT and PDT under single 808 nm laser irradiation.
2：Sample Selection and Data Sources:
The study utilized 4T1 murine breast cancer cell line for in vitro and in vivo experiments. The materials included tungsten chloride, dopamine hydrochloride, and triethylene glycol, among others.
3：List of Experimental Equipment and Materials:
Transmission electron microscopy (TEM, Tecnai G2, FEI, USA), X-ray photoelectron spectroscopy (ESCALAB 250, Thermo Fisher, USA), Malvern Zetasizer Nano-ZS instrument (ZEN3600, Malvern Instruments, USA), UV-2450 spectrophotometer (Shimadzu, Japan), and an 808 nm semiconductor diode laser (Viasho Technology, Beijing, China).
4：Experimental Procedures and Operational Workflow:
The synthesis involved dissolving dopamine hydrochloride and WCl6 in triethylene glycol, heating in a PTFE stainless steel autoclave at 180 °C for 24 h, followed by washing with water and alcohol. Photothermal and photodynamic properties were evaluated under 808 nm laser irradiation.
5：Data Analysis Methods:
The photothermal effect was assessed by temperature variation measurements, and photodynamic activity was evaluated using DPBF as a probe for singlet oxygen generation. Cell viability was determined by MTT assay, and in vivo therapeutic efficacy was assessed by tumor volume measurement and histopathological analysis.
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