研究目的
To identify MALDI-TOF MS biomarkers able to differentiate the subspecies of Staphylococcus schleiferi (S. schleiferi subsp. schleiferi and S. schleiferi subsp. coagulans).
研究成果
The present study identified a set of biomarkers able to discriminate S. schleiferi subspecies by MALDI-TOF mass spectrometry. These biomarkers could help in the determination of novel and improved targets that may assist in the identification of these species/subspecies in clinical samples.
研究不足
The study identified a set of biomarkers for discriminating S. schleiferi subspecies, but further studies are required to improve reproducibility and attest the validity of these biomarkers for this purpose.
1：Experimental Design and Method Selection:
The study used MALDI-TOF MS for the identification of S. schleiferi subspecies.
2：Sample Selection and Data Sources:
Twelve strains (eight S. schleiferi schleiferi and four S. schleiferi coagulans) were selected from a collection of 76 S. schleiferi isolates.
3：List of Experimental Equipment and Materials:
Microflex LT mass spectrometer (Bruker Daltonics), MSP 96 BC target polished steel (Bruker Daltonics, Germany), HCCA matrix (α-cyano-4-hydroxycinnamic acid), formic acid (Sigma-Aldrich), acetonitrile PA (Sigma-Aldrich).
4：Experimental Procedures and Operational Workflow:
Protein extraction protocol was performed, followed by MALDI-TOF MS runs. Spectra were analyzed with BioNumerics software v
5：Data Analysis Methods:
Spectra were processed and analyzed using default parameters, and a set of five MALDI-TOF MS biomarkers were selected to enable the discrimination of S. schleiferi subspecies.
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