研究目的
To develop a highly selective DNA-based electrochemiluminescence (ECL) biosensor for the detection of human IgG, exploiting the effect of steric hindrance to influence the ECL signal in the presence of IgG.
研究成果
A new electrochemiluminescence method for the detection of human IgG based on the steric hindrance effect was successfully developed. The method showed good selectivity, high sensitivity, and a wide linear range, making it feasible for clinical identification of antibodies in various diseases.
研究不足
The study does not mention the application of the biosensor in real samples or its performance in complex biological matrices.
1：Experimental Design and Method Selection:
The biosensor was designed to exploit steric hindrance effects for the detection of human IgG. Europium (II) doped CdSe quantum dots (CdSe:Eu QDs) were used as the ECL signal reporter by combining with signaling DNA.
2：Sample Selection and Data Sources:
Human IgG was used as the target analyte.
3：List of Experimental Equipment and Materials:
The ECL measurement was carried out on a MPI-E detection system. The electrochemical impedance spectroscopy (EIS) measurements and the cyclic voltammetry (CV) analysis were recorded on an Autolab electrochemical analyzer and a CHI660D electrochemical workstation.
4：Experimental Procedures and Operational Workflow:
The gold electrode was modified with capturing DNA, then sealed with MCH, and incubated with signaling DNA-QDs conjugate (S1) including different concentrations of human IgG. ECL detection was accomplished in PBS containing K2S2O
5：Data Analysis Methods:
The ECL intensity was measured and analyzed to determine the concentration of IgG.
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