研究目的
To check the efficacy of the encapsulation on the photosensitizer (PpIX) compared to the naked photosensitizer and to measure the optimal exposure time of measured optimum concentration for the used photosensitizer in photodynamic therapy (PDT) application.
研究成果
The encapsulation by SiNPs greatly increases the ability and the efficiency of PpIX as a photosensitizer to use in PDT. Encapsulated PpIX not only generates singlet oxygen but also destroys the target cells more effectively than naked PpIX, requiring less concentration and exposure time.
研究不足
The cytotoxicity of naked SiNPs and the chemicals used for SiNPs synthesis increased the cytotoxicity, which may affect the efficacy of the encapsulated photosensitizer.
1：Experimental Design and Method Selection:
Reverse-micellar method was used to achieve the encapsulation of PpIX by SiNPs. Different concentrations of PpIX were encapsulated by SiNPs. Osteosarcoma cell line was used in all experiments.
2：Sample Selection and Data Sources:
Osteosarcoma cells were cultured in McCoy’s Medium under appropriate conditions.
3：List of Experimental Equipment and Materials:
Haemocytometer, light source (Intensity =
4：89 mW/cm2 arc lamp), McCoy’s Medium, fetal bovine serum (FBS), antibiotic (Penicillin), Phosphate buffered saline (PBS). Experimental Procedures and Operational Workflow:
Cells were incubated in McCoy’s Medium contains PpIX-SiNPs for 6 h, exposed to light source for 60 min, then washed with PBS and counted.
5：Data Analysis Methods:
The cytotoxicity of the PpIX-SiNPs was measured by using haemocytometer. The percentage of cell death was measured at different concentrations and exposure times.
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