研究目的
To determine whether the cellular physiology of the lens actively maintains the optical properties of the lens and whether inhibition of lens transport affects overall visual quality.
研究成果
The cellular physiology of the lens actively maintains its optical properties and inhibiting the Na/K/ATPase induces a myopic shift in vision similar to that observed clinically in patients who go on to develop cataract.
研究不足
The study is limited to bovine lenses, and the findings may not directly translate to human lenses. The experimental perturbations (high K+ and ouabain) may not fully replicate the physiological changes occurring in human cataract development.
1：Experimental Design and Method Selection:
Bovine lenses were cultured under different conditions to perturb lens physiology, followed by MRI imaging to assess changes in lens geometry, water content, and water/protein ratios. Optical modeling was then used to evaluate the impact on vision quality.
2：Sample Selection and Data Sources:
Fresh bovine eyes were obtained from a local abattoir. Lenses were either imaged intact or dissected for organ culture experiments.
3：List of Experimental Equipment and Materials:
A Varian Unity Inova
4：7 Tesla (T) horizontal bore MRI system was used for imaging. Lenses were cultured in artificial aqueous humor (AAH), AAH-High-K+, or AAH + ouabain. Experimental Procedures and Operational Workflow:
Lenses were incubated for 4 hours under specified conditions, then imaged using MRI to extract geometrical and optical parameters. Optical properties were modeled using ZEMAX software.
5：Data Analysis Methods:
T1 and T2 measurements were used to calculate water content and GRIN. Optical properties were analyzed using ZEMAX to assess changes in optical power and spherical aberration.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
