研究目的
Development of an automated experimental platform for identification of the effects of weak low frequency (LF) electromagnetic field (EMF) on cells.
研究成果
The outputs of this research are expected to reveal new perspectives in the investigation of beneficial effects of LF EMF and their biomedical and biotechnological applications. Developed cell fluctuations measures could reflect viability and metabolic activity of cells.
研究不足
The research faces challenges in finding the weakest MF causing observable effects, the shortest time scale for detecting the effects, and frequency and amplitude windows for biological effects of LF MF.
1：Experimental Design and Method Selection:
The platform comprises multiple measurement stations for monitoring and characterizing cell cultures exposed to weak LF EMF. Methods include electroimpedance spectroscopy (EIS), optical microscopy, and turbidimetry.
2：Sample Selection and Data Sources:
Yeast cell culture (Saccharomyces cerevisiae) is used as a model organism.
3：List of Experimental Equipment and Materials:
Signal generator, 400W power amplifier, low resistive Helmholtz coils, peristaltic circulation pump, portable Stemlab for EIS, inverted light microscopy connected to a digital camera, flow turbidimetry.
4：Experimental Procedures and Operational Workflow:
Cells are exposed to MF in frequency range 0?2 kHz and intensity up to 10 mT. EIS, microscopy, and turbidimetry are used for monitoring.
5：Data Analysis Methods:
Computer analysis of images from video-microscopy, Fourier transform for analyzing cell fluctuations.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容-
signal generator
Generates signals for the exposure system.
-
power amplifier
400W
Amplifies the signal for the Helmholtz coils.
-
Helmholtz coils
low resistive
Generates MF in frequency range 0?2 kHz and intensity up to 10 mT.
-
peristaltic circulation pump
Transports cell suspension through different measurement stations.
-
Stemlab
portable
Measures electric impedance via LAN connectivity.
-
inverted light microscopy
Provides monitoring tools for quantitative characterization of morphology and local kinetics of cells.
-
digital camera
Connected to inverted light microscopy for image capture.
-
flow turbidimetry
Monitors cell density.
-
登录查看剩余6件设备及参数对照表
查看全部
