研究目的
Investigating the method of obtaining large field-of-view super-resolution images by manipulating submerged microspheres.
研究成果
The study demonstrated a simple and efficient method for achieving large field-of-view super-resolution images using a conventional optical microscope combined with a submerged microsphere. The technique, which involves scanning the microsphere across the sample surface and stitching the images, provides a new alternative for super-resolution imaging technology over large areas.
研究不足
The field-of-view of a single microsphere is limited, requiring stitching of multiple images for large area coverage. The method may also be sensitive to the alignment and stability of the microsphere during scanning.
1:Experimental Design and Method Selection:
The study used a four-dimensional precision translation stage to position a microsphere for super-resolution imaging. Simulations were performed using the finite element method (FEM) in COMSOL multiphysics software to select the appropriate microsphere size and liquid medium.
2:Sample Selection and Data Sources:
The sample was a Blu-ray disc with nano-features of 220 nm wide stripes spaced by 130 nm groves. The simulations and experiments focused on the imaging quality obtained by different microsphere sizes in various liquids.
3:List of Experimental Equipment and Materials:
Equipment included a professional probe station (M150, Cascade Microtech), an optical microscope (PS-888 Microscope, SEIWA OPTICAL), a white light source (halogen lamp, LampLink2, OPTEM), a 50X objective lens (0.42 NA, M Plan Apo, SEIWA OPTICAL), and a colour charge-coupled device (CCD) camera (FL3-U3-13S2C-CS, point grey). Materials included barium titanate glass (BTG) microspheres, alcohol, and Cedarwood oil.
4:42 NA, M Plan Apo, SEIWA OPTICAL), and a colour charge-coupled device (CCD) camera (FL3-U3-13S2C-CS, point grey). Materials included barium titanate glass (BTG) microspheres, alcohol, and Cedarwood oil. Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: The microsphere was attached to a probe and submerged in alcohol. The translation stage was used to scan the microsphere across the sample surface, and images were recorded by the CCD camera. Large field-of-view images were obtained by stitching the recorded images.
5:Data Analysis Methods:
The quality of the images was analyzed based on the resolution and contrast, with simulations confirming the negligible influence of the probe and flowing liquid on imaging.
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probe station
M150
Cascade Microtech
Used for positioning and scanning the microsphere across the sample surface.
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optical microscope
PS-888 Microscope
SEIWA OPTICAL
Used for observing the sample and recording the super-resolution images.
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white light source
LampLink2
OPTEM
Provided illumination for the imaging process.
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objective lens
M Plan Apo
SEIWA OPTICAL
Used for magnifying the image of the sample.
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CCD camera
FL3-U3-13S2C-CS
point grey
Recorded the images obtained through the microsphere.
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probe
ST-20-2
Picoprobe
Used to attach and position the microsphere.
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translation stage
KS102-70R
SURUGA SEIKI
Used for precise movement of the microsphere along the X and Y axes.
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Z axis
B31-80A
SURUGA SEIKI
Used for changing the distance between the sample surface and the sphere.
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rotation stage
DCM 210
Cascade Microtech
Used for adjusting the probe angle.
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